[比较实时逆转录聚合酶链反应与两种培养方法用于流感病毒检测的效果]
[Compare real-time RT-PCR with two culture methods for influenza virus detection].
作者信息
Li Jian-xiong, Fang Shi-song, Cheng Xiao-wen, Wang Ting, Wang Xin, Lv Xing, Wu Chun-li, Zhang Ren-li, Cheng Jin-quan, Yu Mu-hua
机构信息
School of Public Health, Sun Yat-Sen University, Guangzhou 510080, China.
出版信息
Zhonghua Shi Yan He Lin Chuang Bing Du Xue Za Zhi. 2011 Feb;25(1):66-8.
OBJECTIVE
Real-time RT-PCR, cell culture and embryonated eggs culture for influenza detection were compared by analyzing the data of influenza surveillance in Shenzhen in second half of 2009.
METHODS
1092 clinical samples (throat swabs) collected during second half of 2009 were tested by real-time RT-PCR, cell culture and embryonated eggs culture, and the results were analyzed by statistical methods.
RESULTS
The positive rate were 54.21%, 27.11% and 16.21% using real-time RT-PCR, cell culture and embryonated eggs culture, and the sensitive were 100%, 50% and 29.9%. The lowest dilutions of virus detected by real-time RT-PCR were 10(-2) TCID50/ml.
CONCLUSION
The sensitive of real-time RT-PCR was higher than culture and the specificity was also very high. It was more suitable for emergency detect. The sensitive of cell culture for H3N2 subtype was higher, and sensitive of embryonated eggs culture for type B was higher.
目的
通过分析2009年下半年深圳流感监测数据，比较实时荧光定量逆转录聚合酶链反应（Real-time RT-PCR）、细胞培养和鸡胚培养用于流感检测的情况。
方法
对2009年下半年采集的1092份临床样本（咽拭子）分别采用实时荧光定量逆转录聚合酶链反应、细胞培养和鸡胚培养进行检测，并对结果进行统计学分析。
结果
实时荧光定量逆转录聚合酶链反应、细胞培养和鸡胚培养的阳性率分别为54.21%、27.11%和16.21%，敏感度分别为100%、50%和29.9%。实时荧光定量逆转录聚合酶链反应检测到病毒的最低稀释度为10（-2）TCID50/ml。
结论
实时荧光定量逆转录聚合酶链反应的敏感度高于培养法，特异性也很高，更适合应急检测。细胞培养对H3N2亚型的敏感度较高，鸡胚培养对B型的敏感度较高。
Zotero 插件