Vydriakova G A, Gusev A A, Medvedeva S E
Prikl Biokhim Mikrobiol. 2011 May-Jun;47(3):324-9.
The possibility of the development of the solid phase bioluminescent biotest using aerial mycelium of the luminous fungi was investigated. Effect of organic and inorganic toxic compounds (TC) at concentrations from 10(-6) to 1 mg/ml on luminescence of aerial mycelia of four species of luminous fungi-Armillaria borealis (Culture Collection of the Institute of Forest, Siberian Branch, Russian Academy of Sciences), A. mellea, A. gallica, and Lampteromyces japonicus (Fungi Collection of the Botanical Institute, Russian Academy of Sciences)--has been studied. Culture of A. mellea was shown to be most sensitive to solutions of the model TC. It was demonstrated that the sensitivity of the luminous fungi is comparable with the sensitivity of the bacteria that are used for environmental monitoring. Use of the aerial mycelium of the luminous fungi on the solid support as a test object is a promising approach in biotesting for the development of continuous biosensors for air monitoring.
研究了利用发光真菌气生菌丝体开发固相生物发光生物测试的可能性。研究了浓度为10(-6)至1毫克/毫升的有机和无机有毒化合物（TC）对四种发光真菌——北方蜜环菌（俄罗斯科学院西伯利亚分院森林研究所菌种保藏中心）、蜜环菌、高卢蜜环菌和日本发光小菇（俄罗斯科学院植物研究所真菌保藏中心）气生菌丝体发光的影响。结果表明，蜜环菌培养物对模型TC溶液最为敏感。结果表明，发光真菌的敏感性与用于环境监测的细菌的敏感性相当。将发光真菌的气生菌丝体用作固体支持物上的测试对象，是开发用于空气监测的连续生物传感器的生物测试中有前景的方法。
