Olives J P, Voigt J J, al Saati T, Nonnenmacher L, Brousset P, Delsol G, Ghisolfi J
Service de Médecine Infantile D, Gastroentérologie et Nutrition, CHU Purpan, Toulouse.
Gastroenterol Clin Biol. 1990;14(1):33-40.
In order to study the distribution of lymphocyte subpopulations in a pathologic intestinal mucosa, the authors, instead of using the classic method by counting the number of lymphocytes, present an original method permitting the exploitation of quantified data from labelled surface cells by texture analyser coupled with a computerized system. We investigated 25 children presenting with chronic diarrhea and villous atrophy and 5 control subjects. Fifteen of the 25 children had celiac disease (10 active with total villous atrophy and 5, celiac disease in remission with healing mucosa), 5 cow's milk protein intolerance with total or partial villous atrophy and 5, chronic diarrhea with partial villous atrophy. Immunohistochemical study with monoclonal antibodies was carried out on frozen sections using a three-step immunoperoxidase technique. Compared with the 5 controls, patients with food intolerance (celiac disease and cow's milk protein intolerance) showed a significant increase of T suppressor lymphocytes (p less than 0.01 and p less than 0.05) in the epithelium, whereas there were more T helper lymphocytes in the lamina propria (p less than 0.05 and p less than 0.01). Non-treated celiac disease was distinguished from treated celiac disease by a marked increase in intra-epithelial T cytotoxic-suppressors. These results suggest that T cytotoxic-suppressors may be the mediators of the lesions observed in celiac disease.
为了研究病理性肠黏膜中淋巴细胞亚群的分布情况，作者未采用通过计数淋巴细胞数量的经典方法，而是提出了一种原始方法，该方法允许通过纹理分析仪结合计算机系统利用标记表面细胞的量化数据。我们研究了25例患有慢性腹泻和绒毛萎缩的儿童以及5名对照受试者。25例儿童中，15例患有乳糜泻（10例处于活动期且伴有完全绒毛萎缩，5例乳糜泻缓解期且黏膜愈合），5例对牛奶蛋白不耐受伴有完全或部分绒毛萎缩，5例慢性腹泻伴有部分绒毛萎缩。使用三步免疫过氧化物酶技术对冰冻切片进行单克隆抗体免疫组织化学研究。与5名对照相比，食物不耐受患者（乳糜泻和牛奶蛋白不耐受）上皮中的抑制性T淋巴细胞显著增加（p<0.01和p<0.05），而固有层中的辅助性T淋巴细胞更多（p<0.05和p<0.01）。未经治疗的乳糜泻与经治疗的乳糜泻的区别在于上皮内细胞毒性抑制性T细胞显著增加。这些结果表明，细胞毒性抑制性T细胞可能是乳糜泻中观察到的病变的介导因素。
