Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100193, China.
Food Addit Contam Part A Chem Anal Control Expo Risk Assess. 2011 Aug;28(8):1091-7. doi: 10.1080/19440049.2011.576443. Epub 2011 Jun 22.
Fungi associated with ochratoxin A (OTA)-contaminated liquorice root and their capabilities for OTA production were investigated. Medicinal materials of mouldy liquorice root were collected from herbal markets located in Jiangxi, Zhejiang, Henan provinces and Beijing, China, respectively. Sixteen fungal species belonging to Penicillium, Aspergillus, Eurotium, Fusarium, Mucor and Scopulariopsis were isolated; the fungal composition was different in each liquorice root sample. Penicillium polonicum was predominant, comprising 54% of the total isolates in the liquorice root sample from Jiangxi province, which was contaminated with OTA at the highest level. In other samples with lower OTA contents, species of Aspergillus and Eurotium were predominant. OTA production of representative strains on rice media was detected by LC-MS/MS; all Penicillium polonicum isolates and a P. chrysogenum were ochratoxigenic; OTA concentrations ranged from 6.94 to 217.37 ng g(-1). This is the first study to report P. polonicum as an OTA-producing fungus. OTA contamination of mouldy liquorice root constitutes a major health hazard in consumption. This situation demands urgent and undivided attention.
本研究调查了与赭曲霉毒素 A（OTA）污染的甘草根相关的真菌及其产生 OTA 的能力。分别从中国江西、浙江、河南和北京的草药市场采集了霉变甘草根的药材。从霉变甘草根样本中分离出了 16 种真菌，分别属于青霉属、曲霉属、帚霉属、镰刀菌属、毛霉属和枝孢属；每个甘草根样本中的真菌组成均不同。在江西的样本中，波兰青霉占优势，占总分离物的 54%，该样本中的 OTA 污染水平最高。在其他 OTA 含量较低的样本中，曲霉属和帚霉属的物种占优势。通过 LC-MS/MS 检测了代表性菌株在大米培养基上的 OTA 产生情况；所有波兰青霉分离株和一株黄曲霉均产 OTA；OTA 浓度范围为 6.94 至 217.37ng/g。这是首次报道波兰青霉是产 OTA 的真菌。霉变甘草根的 OTA 污染对食用构成了重大健康危害。这种情况需要紧急和一致的关注。
