Fenwal, Inc., Lake Zurich, Illinois 60047, USA.
Transfusion. 2012 Jan;52(1):188-94. doi: 10.1111/j.1537-2995.2011.03252.x. Epub 2011 Jul 25.
Platelet additive solutions (PASs) facilitate improved recovery of plasma and may reduce the severity and/or frequency of plasma-associated transfusion reactions. Current apheresis platelet (PLT) PAS products contain approximately 30 to 40% residual plasma. In an effort to further decrease the residual plasma, two in vitro studies were conducted with PLTs suspended in 5% plasma and a reformulated PAS-3, named PAS-5, that contains additional salts, glucose, and bicarbonate.
In Study 1, PLTs suspended in 5% plasma/95% PAS-5 were prepared directly on a separator (Amicus, Fenwal, Inc.) without additional centrifugation or washing. In Study 2, a double unit of hyperconcentrated Amicus PLTs in plasma was collected, divided, and centrifuged to prepare a control unit in 100% plasma and a paired test unit in 5% plasma/95% PAS-5. The in vitro properties of PLTs were assessed in both studies during 7-day storage at 20 to 24°C with continuous agitation.
In Study 1, PLT concentration, pH, mean PLT volume (MPV), HCO(3)(-), pCO(2), pO(2), lactate dehydrogenase, and hypotonic shock response (HSR) did not significantly change during storage. By Day 7, glucose levels and morphology scores modestly decreased (17.6 and 14.4%, respectively) and lactate levels modestly increased (to 7.2 mmol/L). In Study 2, MPV, pH, glucose, pO(2), HSR, and morphology were comparable in control and test PLTs during 7-day storage. Glucose consumption and lactate production were significantly less in test versus control PLTs (p≤0.0015). Extent of shape change and %CD62P-positive test PLTs were less than those of controls (p<0.001).
Apheresis PLTs suspended in 5% plasma/95% PAS-5 maintained in vitro properties during 7-day storage.
血小板添加剂溶液 (PAS) 有助于提高血浆的回收率，并可能降低血浆相关输血反应的严重程度和/或频率。目前的单采血小板 (PLT) PAS 产品含有约 30%至 40%的残留血浆。为了进一步降低残留血浆，对悬浮在 5%血浆中的 PLT 进行了两项体外研究，并对一种新配方的 PAS-3（命名为 PAS-5）进行了研究，该配方含有额外的盐、葡萄糖和碳酸氢盐。
在研究 1 中，直接在分离器 (Amicus，Fenwal，Inc.) 上制备悬浮在 5%血浆/95% PAS-5 中的 PLT，无需额外的离心或洗涤。在研究 2 中，收集了两单位的高浓度 Amicus PLT 在血浆中，将其分开并离心，以制备 100%血浆中的对照单位和 5%血浆/95% PAS-5 中的配对测试单位。在两项研究中，在 20 至 24°C 下连续搅拌的 7 天储存期间评估了 PLT 的体外特性。
在研究 1 中，PLT 浓度、pH 值、平均 PLT 体积 (MPV)、HCO(3)(-)、pCO(2)、pO(2)、乳酸脱氢酶和低渗休克反应 (HSR) 在储存过程中没有明显变化。到第 7 天，葡萄糖水平和形态评分略有下降（分别为 17.6%和 14.4%），乳酸水平略有升高（至 7.2mmol/L）。在研究 2 中，在 7 天储存期间，对照和测试 PLT 的 MPV、pH 值、葡萄糖、pO(2)、HSR 和形态相似。与对照 PLT 相比，测试 PLT 的葡萄糖消耗和乳酸生成明显减少（p≤0.0015）。测试 PLT 的形状变化程度和%CD62P 阳性 PLT 均低于对照（p<0.001）。
悬浮在 5%血浆/95% PAS-5 中的单采 PLT 在 7 天储存期间保持体外特性。
