Section of Endocrinology, Andrology and Internal Medicine, Department of Internal Medicine and Systemic Diseases, University of Catania, Italy.
Andrologia. 2012 Jun;44(3):152-6. doi: 10.1111/j.1439-0272.2010.01124.x. Epub 2011 Jul 25.
Platelet activation results from the exposure of receptors on the surface of the platelet to specific structures on the vessel wall. The aim of this study was to assess the serum concentrations of apoptotic endothelial microparticles (EMPa) and the vitronectin receptor (VR) in patients with erectile dysfunction (ED) before and after treatment with tadalafil. This study included 50 patients with arterial ED. EMPa and VR levels were measured by using flow cytometry. The CD45(neg)-CD144(pos)-annexin V(Pos) events were defined as EMPa, and the CD51(pos)-CD61(pos) events were defined as VR. Patients with ED were evaluated before and after daily treatment with 5 mg tadalafil for 90 days. Patients with arterial ED had serum concentrations of EMPa and VR significantly higher than the control group at baseline. After tadalafil, the serum concentrations of EMPa and VR of the patients with arterial ED were significantly lower than before treatment, but significantly higher than controls. Patients with arterial ED expressed higher levels of both EMPa that are associated with dysfunction of the arterial endothelial pathway and VR-expressing platelet-endothelial elements. Chronic treatment with tadalafil reduced endothelial apoptosis in these patients and also reduced VR expression.
血小板的激活源于其表面受体与血管壁上特定结构的结合。本研究旨在评估勃起功能障碍（ED）患者在接受他达拉非治疗前后血清中凋亡内皮微颗粒（EMPa）和玻璃粘连蛋白受体（VR）的浓度。本研究纳入了 50 例动脉性 ED 患者。采用流式细胞术检测 EMPa 和 VR 水平。CD45(neg)-CD144(pos)-annexin V(Pos)事件被定义为 EMPa，CD51(pos)-CD61(pos)事件被定义为 VR。ED 患者接受每日 5mg 他达拉非治疗 90 天。与对照组相比，动脉性 ED 患者的血清 EMPa 和 VR 浓度在基线时明显升高。经他达拉非治疗后，动脉性 ED 患者的血清 EMPa 和 VR 浓度明显低于治疗前，但明显高于对照组。动脉性 ED 患者表达了更高水平的 EMPa，这与动脉内皮途径功能障碍有关，同时也表达了 VR 阳性的血小板内皮细胞成分。慢性他达拉非治疗降低了这些患者的内皮细胞凋亡，同时也降低了 VR 的表达。
