La Vignera Sandro, Condorelli Rosita, Vicari Enzo, D'Agata Rosario, Calogero Aldo E
Section of Endocrinology, Andrology, and Internal Medicine, Department of Internal Medicine and Systemic Diseases, Catania University, Policlinico G. Rodolico, S Sofia 78th St, Bldg 4, Rm 2C82, 95123 Catania, Italy.
J Androl. 2012 Mar-Apr;33(2):202-9. doi: 10.2164/jandrol.111.013136. Epub 2011 Apr 7.
Blood endothelial progenitor cells (EPC) and microparticles (EMP) have been proposed as markers of endothelial dysfunction. The aim of this study was to evaluate both EPCs and EMPs in patients with arterial erectile dysfunction (ED) and metabolic syndrome (MetS). To accomplish this, 100 patients (ages 45-60 years) with ED and MetS (Adult Treatment Panel III [ATP III] 1999 criteria) and 17 healthy men (ages 44-57 years) were selected. EPC (CD45(neg)/CD34(pos)/CD144(pos)) and EMP (CD45(neg)/CD144(pos)/Annexin V(pos)) blood concentrations were evaluated by flow cytometry, before and after administration of tadalafil (20 mg) on demand for 3 months. Before treatment, EPCs and EMPs were significantly higher in patients compared with healthy men. EPCs increased significantly after tadalafil administration, whereas EMPs did not differ significantly. EPCs correlated positively or negatively with body mass index and with some cavernous artery indices, both before and after tadalafil administration. EMPs showed only positive correlations with body mass index and some cavernous artery indices, both before and after tadalafil administration. Patients with arterial ED and MetS have higher EPCs and EMPs compared with healthy men; hence, these cells may be regarded as markers of cavernous artery dysfunction. Tadalafil administration increased EPCs but not EMPs, suggesting that this compound may play a role in the endothelial repair response.
血液内皮祖细胞（EPC）和微粒（EMP）已被提出作为内皮功能障碍的标志物。本研究的目的是评估动脉性勃起功能障碍（ED）和代谢综合征（MetS）患者的EPC和EMP。为实现这一目的，选取了100例年龄在45至60岁之间、患有ED和MetS（符合成人治疗小组第三次报告[ATP III]1999年标准）的患者以及17名年龄在44至57岁之间的健康男性。通过流式细胞术评估按需服用他达拉非（20毫克）3个月前后EPC（CD45阴性/CD34阳性/CD144阳性）和EMP（CD45阴性/CD144阳性/膜联蛋白V阳性）的血液浓度。治疗前，患者的EPC和EMP显著高于健康男性。服用他达拉非后EPC显著增加，而EMP无显著差异。在服用他达拉非前后，EPC与体重指数以及一些海绵体动脉指标呈正相关或负相关。EMP仅与体重指数以及一些海绵体动脉指标呈正相关，无论在服用他达拉非前后。与健康男性相比，患有动脉性ED和MetS的患者具有更高的EPC和EMP；因此，这些细胞可被视为海绵体动脉功能障碍的标志物。服用他达拉非可增加EPC但不增加EMP，表明该化合物可能在内皮修复反应中发挥作用。
