Noschka E, Werre S R, Crisman M V, Thatcher C D, Milne G L, Dahlgren L A
School of Animal and Veterinary Sciences, The University of Adelaide, Australia.
Equine Vet J Suppl. 2011 Aug(39):34-41. doi: 10.1111/j.2042-3306.2011.00384.x.
F2-isoprostanes have been used extensively to quantify lipid peroxidation in association with risk factors in various diseases. Horses with colic may have intestinal ischaemia and/or inflammation characterised by oxidative stress and increased production of isoprostanes.
To gather preliminary data regarding the feasibility of using urine F2-isoprostanes and isoprostane metabolites as early screening tools for the presence of gastrointestinal disease requiring surgical intervention in horses and ultimately develop a stall-side test capable of identifying these horses as early as possible for timely referral.
Concentrations of urine isoprostane and isoprostane metabolite were determined by mass spectroscopy and normalised to urine creatinine (Cr) concentrations in urine samples from 42 healthy control horses and 43 horses with gastrointestinal pain or colic.
Horses with colic were treated medically (n = 21) or surgically (n = 22). Mean ± s.d. concentrations of urine isoprostane and isoprostane metabolite were significantly higher in horses with colic (2.94 ± 1.69 and 0.31 ± 0.22 ng/mg Cr, respectively), compared to control horses (1.89 ± 1.39 and 0.22 ± 0.08 ng/mg Cr, respectively). Mean urine isoprostane metabolite concentrations were significantly higher in horses undergoing surgery (0.38 ± 0.28 ng/mg Cr) compared to controls and medical colics (0.26 ± 0.11 ng/mg Cr). Nonsurvivors had significantly higher mean urine isoprostane metabolite concentrations (0.47 ± 0.39 ng/mg Cr) than control or surviving colic horses (0.29 ± 0.24 ng/mg Cr).
Measurement of urine isoprostane metabolite concentration may be a useful prognostic indicator in equine colic.
Urine isoprostane metabolites may aid in early recognition of surgical colic. Isoprostanes are a potential therapeutic target to prevent further systemic and gastrointestinal tissue injury in horses with colic.
F2-异前列腺素已被广泛用于量化与各种疾病风险因素相关的脂质过氧化。患有绞痛的马匹可能存在肠道缺血和/或炎症，其特征为氧化应激和异前列腺素生成增加。
收集关于使用尿液F2-异前列腺素和异前列腺素代谢物作为早期筛查工具来检测需要手术干预的马匹胃肠道疾病的可行性的初步数据，并最终开发一种能够尽早识别这些马匹以便及时转诊的现场检测方法。
通过质谱法测定42匹健康对照马和43匹患有胃肠道疼痛或绞痛的马的尿液样本中异前列腺素和异前列腺素代谢物的浓度，并将其标准化为尿液肌酐（Cr）浓度。
患有绞痛的马接受了药物治疗（n = 21）或手术治疗（n = 22）。与对照马（分别为1.89±1.39和0.22±0.08 ng/mg Cr）相比，患有绞痛的马尿液中异前列腺素和异前列腺素代谢物的平均±标准差浓度显著更高（分别为2.94±1.69和0.31±0.22 ng/mg Cr）。与对照马和药物治疗的绞痛马（0.26±0.11 ng/mg Cr）相比，接受手术的马尿液中异前列腺素代谢物的平均浓度显著更高（0.38±0.28 ng/mg Cr）。非存活者尿液中异前列腺素代谢物的平均浓度（0.47±0.39 ng/mg Cr）显著高于对照马或存活的绞痛马（0.29±0.24 ng/mg Cr）。
测定尿液中异前列腺素代谢物浓度可能是马绞痛的一个有用的预后指标。
尿液异前列腺素代谢物可能有助于早期识别手术性绞痛。异前列腺素是预防患有绞痛的马进一步发生全身和胃肠道组织损伤的潜在治疗靶点。
