Morton A J, Grosche A, Matyjaszek S A, Polyak M M R, Freeman D E
Island Whirl Equine Colic Research Laboratory, Department of Large Animal Clinical Sciences, College of Veterinary Medicine, University of Florida, FL, USA.
Equine Vet J Suppl. 2011 Aug(39):112-6. doi: 10.1111/j.2042-3306.2011.00382.x.
The effects of prostaglandins and nonsteroidal anti-inflammatory drugs (NSAIDs) on repair of equine intestinal mucosa are important since most horses with gastrointestinal diseases are routinely treated with NSAIDs, such as flunixin meglumine (FM), and these drugs can be toxic to equine gastrointestinal mucosa.
Flunixin meglumine would not affect recovery of equine colonic mucosa in vitro, 18 h after a reversible ischaemic injury.
In 14 anaesthetised horses, a segment of pelvic flexure was subjected to 2 h of ischaemia and the horses were allowed to recover for 18 h. Seven horses received normal saline and 7 received FM, 1.1 mg/kg bwt i.v., at the end of ischaemia and 12 h later. Colonic mucosa was harvested during a second anaesthesia, 18 h after recovery from ischaemia and then horses were subjected to euthanasia. Transepithelial electrical resistance (TER) and transepithelial flux of tritiated mannitol were used to measure mucosal permeability during 4 h of incubation in Ussing chambers, with the following in vitro treatments: 1) no addition, 2) FM 14 µmol/l as powder, 3) FM 14 µmol/l in injectable form and 4) diluent for injectable FM. Histomorphological changes were assessed by light microscopy.
There were no significant differences in any of the measurements between saline and FM treated horses. The mucosal height of the ischaemic FM tissues incubated in diluent was significantly decreased compared to the nonischaemic tissues.
Flunixin meglumine did not adversely affect barrier integrity in ischaemic equine colonic mucosa.
前列腺素和非甾体抗炎药（NSAIDs）对马肠道黏膜修复的影响至关重要，因为大多数患有胃肠道疾病的马通常会接受NSAIDs治疗，如氟尼辛葡甲胺（FM），而这些药物可能对马的胃肠道黏膜有毒性。
在可逆性缺血损伤18小时后，氟尼辛葡甲胺不会影响马结肠黏膜在体外的恢复。
对14匹麻醉的马，一段骨盆曲部进行2小时的缺血处理，然后让马恢复18小时。7匹马在缺血结束时静脉注射生理盐水，7匹马在缺血结束时静脉注射1.1mg/kg体重的FM，并在12小时后再次注射。在从缺血恢复18小时后的第二次麻醉期间采集结肠黏膜，然后对马实施安乐死。在Ussing室中孵育4小时期间，使用跨上皮电阻（TER）和氚标记甘露醇的跨上皮通量来测量黏膜通透性，采用以下体外处理方法：1）不添加任何物质，2）14µmol/l粉末状FM，3）14µmol/l注射用FM，4）注射用FM的稀释剂。通过光学显微镜评估组织形态学变化。
生理盐水处理组和FM处理组之间的任何测量值均无显著差异。与非缺血组织相比，在稀释剂中孵育的缺血FM组织的黏膜高度显著降低。
氟尼辛葡甲胺对缺血马结肠黏膜的屏障完整性没有不利影响。
