Freeman D E, Inoue O J, Eurell T E
Department of Veterinary Clinical Medicine, College of Veterinary Medicine, University of Illinois, Urbana 61801, USA.
Am J Vet Res. 1997 Aug;58(8):915-9.
To study the effect of flunixin meglumine on short circuit current (Isc) in equine right ventral colon in vitro.
Intestinal mucosa from healthy horses and ponies.
Isc was measured in mucosa from the right ventral colon mounted in Ussing chambers. In experiment 1, collection and incubation solutions were: control (no additions); flunixin meglumine, 4 micrograms/ml; indomethacin, 10(-6) M; and flunixin meglumine (4 micrograms/ml) with 10(-6) M prostaglandin E2. In experiment 2, incubation conditions were: control [plain Krebs-Ringer bicarbonate [KRB] solution]; flunixin meglumine, 4 micrograms/ml in KRB; chloride-free buffer solution; flunixin meglumine (4 micrograms/ml) in a chloride-free buffer solution; and plain KRB with 10(-6) M prostaglandin E2. In experiment 3, tissue from 3 groups (n = 6 each) of animals: controls, physiologic saline solution given IV at 10 minutes before euthanasia; flunixin meglumine (1.1 mg/kg of body weight, IV) given at 10 minutes before euthanasia; and treatment similar to controls, except that tissues were incubated with 8 micrograms of flunixin meglumine/ml of bathing medium.
Flunixin meglumine and indomethacin reduced Isc to approximately a third of control current (P < 0.05), but coincubation with flunixin meglumine and 10(-5) M prostaglandin E2 restored Isc close to the control value. Incubation with 10(-6) M prostaglandin E2 alone did not change Isc. When chloride was substituted with isethionate, flunixin meglumine had no effect on Isc. Flunixin meglumine given before euthanasia or included at a concentration of 8 micrograms/ml in all tissue preparation and incubation solutions reduced Isc (P < 0.05).
Flunixin meglumine given IV or added to bathing solutions decreased Isc in equine right ventral colon by a mechanism that appeared to involve prostaglandin-mediated chloride secretion.
Our results suggest that flunixin meglumine given IV to horses at recommended doses could alter putative effects of colonic prostaglandins.
研究氟尼辛葡甲胺对体外培养的马右侧腹结肠短路电流（Isc）的影响。
健康马和矮种马的肠黏膜。
将右侧腹结肠黏膜安装在尤斯灌流小室中测量Isc。在实验1中，采集和孵育溶液分别为：对照（无添加物）；氟尼辛葡甲胺，4微克/毫升；吲哚美辛，10⁻⁶摩尔/升；以及氟尼辛葡甲胺（4微克/毫升）与10⁻⁵摩尔/升前列腺素E2。在实验2中，孵育条件为：对照[普通碳酸氢盐克氏林格液（KRB）]；KRB中氟尼辛葡甲胺，4微克/毫升；无氯缓冲液；无氯缓冲液中氟尼辛葡甲胺（4微克/毫升）；以及含10⁻⁶摩尔/升前列腺素E2的普通KRB。在实验3中，取3组动物（每组n = 6）的组织：对照组，安乐死前10分钟静脉注射生理盐水；安乐死前10分钟静脉注射氟尼辛葡甲胺（1.1毫克/千克体重）；处理方式与对照组相似，但组织在含8微克/毫升氟尼辛葡甲胺的孵育液中孵育。
氟尼辛葡甲胺和吲哚美辛将Isc降低至对照电流的约三分之一（P < 0.05），但氟尼辛葡甲胺与10⁻⁵摩尔/升前列腺素E2共同孵育可使Isc恢复至接近对照值。单独用10⁻⁶摩尔/升前列腺素E2孵育不改变Isc。当用羟乙磺酸盐替代氯化物时，氟尼辛葡甲胺对Isc无影响。安乐死前给予氟尼辛葡甲胺或在所有组织制备和孵育溶液中加入浓度为8微克/毫升的氟尼辛葡甲胺可降低Isc（P < 0.05）。
静脉注射或添加到孵育液中的氟尼辛葡甲胺可降低马右侧腹结肠的Isc，其机制似乎涉及前列腺素介导的氯分泌。
我们的结果表明，以推荐剂量给马静脉注射氟尼辛葡甲胺可能会改变结肠前列腺素的假定作用。
