薄荷油促进肝胆汁分泌作用的初步实验研究。
Preliminary experimental research on the mechanism of liver bile secretion stimulated by peppermint oil.
机构信息
Department of Gastroenterology, Shanghai First People's Hospital, Shanghai Jiaotong University, Shanghai, China.
出版信息
J Dig Dis. 2011 Aug;12(4):295-301. doi: 10.1111/j.1751-2980.2011.00513.x.
OBJECTIVE
To investigate the choleretic effect and molecular mechanisms of action of peppermint oil (PO), the main component of Danshu capsules (Sichuan Jishengtang Pharmaceutical Co., Ltd., Pengzhou, Sichuan Province, China).
METHODS
Bile secretion was measured by biliary drainage in rats. Total bile acids, total cholesterol and bilirubin in bile were determined. Cholesterol 7α-hydroxylase (CYP7A1), and farnesoid X receptor (FXR) messenger ribonucleic acid (mRNA) levels were assessed in HepG2 cells (a human hepatocellular carcinoma cell line) by reverse transcription polymerase chain reaction (RT-PCR).
RESULTS
PO significantly promoted bile and bile acid secretion in rats. It also increased bile acid efflux and decreased cholesterol levels (P < 0.01) in bile. In HepG2 cells the mRNA levels of CYP7A1 and FXR were significantly upregulated after treatment with PO.
CONCLUSIONS
PO stimulates bile fluid secretion and thus has a choleretic effect. PO might play a role in upregulating CYP7A1 and FXR mRNA levels, suggesting that the molecular mechanisms are related to gene expression involved in bile acid synthesis.
目的
研究薄荷油（PO）的利胆作用及其作用机制，PO 是丹术胶囊（四川济生堂药业有限公司，四川彭州）的主要成分。
方法
通过大鼠胆汁引流法测定胆汁分泌量，测定胆汁中总胆汁酸、总胆固醇和胆红素的含量。采用逆转录聚合酶链反应（RT-PCR）法检测 HepG2 细胞（人肝癌细胞系）中胆固醇 7α-羟化酶（CYP7A1）和法尼醇 X 受体（FXR）信使核糖核酸（mRNA）的水平。
结果
PO 能显著促进大鼠胆汁和胆汁酸的分泌，增加胆汁酸的流出，降低胆汁中的胆固醇水平（P<0.01）。PO 处理后 HepG2 细胞中 CYP7A1 和 FXR 的 mRNA 水平明显上调。
结论
PO 刺激胆汁液分泌，具有利胆作用。PO 可能通过上调 CYP7A1 和 FXR mRNA 水平发挥作用，提示其分子机制与涉及胆汁酸合成的基因表达有关。
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