Department of Oral Pharmacology, School of Dentistry and Institute of Dental Bioscience, BK21 project, Chonbuk National University, Jeonju (651-756), Korea.
Graduate School of Life Science, CHA Stem Cell Institute, College of Medicine, CHA University, 605-21 Yeoksam 1 dong, Gangnam gu, Seoul 135-907, Korea.
Proteome Sci. 2011 Jul 26;9:41. doi: 10.1186/1477-5956-9-41.
Many important molecular events associated with implantation and development occur within the female reproductive tract, especially within the uterus endometrium, during pregnancy periods. The endometrium includes the mucosal lining of the uterus, which provides a suitable site for implantation and development of a fertilized egg and fetus. To date, the molecular cascades in the uterus endometrium during pregnancy periods in pigs have not been elucidated fully. In this study, we compared the functional regulated proteins in the endometrium during pregnancy periods with those in non-pregnant conditions and investigated changes in expression patterns during pregnancy (days 40, 70, and 93) using two-dimensional gel electrophoresis (2-DE) and western blotting. The functional regulated proteins were identified and discovered from differentially expressed proteins in the uterus endometrium during pregnancy. We discovered 820 protein spots in a proteomic analysis of uterus endometrium tissues with 2-DE gels. We identified 63 of the 98 proteins regulated differentially among non-pregnant and pregnant tissues (matched and unmatched spots). Interestingly, 10 of these 63 proteins are development-, cytoskeleton- and chaperon-related proteins such as transferrin, protein DJ-1, transgelin, galectin-1, septin 2, stathmin 1, cofilin 1, fascin 1, heat shock protein (HSP) 90β and HSP 27. The specific expression patterns of these proteins in the endometrium during pregnancy were confirmed by western blotting. Our results suggest that the expressions of these genes involved in endometrium function and endometrium development from early to late gestation are associated with the regulation of endometrium development for maintaining pregnancy.
许多与着床和发育相关的重要分子事件发生在女性生殖器官内，特别是在怀孕期间的子宫子宫内膜中。子宫内膜包括子宫的黏膜衬里，为受精卵和胎儿的着床和发育提供了合适的场所。迄今为止，猪在怀孕期间子宫子宫内膜中的分子级联反应尚未完全阐明。在这项研究中，我们比较了妊娠期间和非妊娠期间子宫内膜中的功能调节蛋白，并通过二维凝胶电泳（2-DE）和 Western blot 分析了妊娠期间（第 40、70 和 93 天）的表达模式变化。通过对妊娠期间子宫子宫内膜中的差异表达蛋白进行功能调节蛋白的鉴定和发现。我们在子宫内膜的蛋白质组学分析中发现了 2-DE 凝胶中的 820 个蛋白斑点。我们在非妊娠和妊娠组织（匹配和不匹配的斑点）之间鉴定出了 63 种差异调节的蛋白质。有趣的是，这 63 种蛋白质中有 10 种是与发育、细胞骨架和伴侣相关的蛋白质，如转铁蛋白、DJ-1 蛋白、转胶蛋白、半乳糖凝集素-1、隔蛋白 2、Stathmin 1、原肌球蛋白 1、丝束蛋白 1、热休克蛋白（HSP）90β 和 HSP 27。通过 Western blot 验证了这些蛋白质在妊娠期间子宫内膜中的特定表达模式。我们的结果表明，这些参与子宫内膜功能和子宫内膜发育的基因的表达与维持妊娠的子宫内膜发育调节有关。
