Gou Jinhai, Jia Jia, Zhao Xia, Yi Tao, Li Zhengyu
Department of Gynecology and Obstetrics, West China Second University Hospital, Sichuan University, Chengdu 610041, People's Republic of China.
Department of Gynecology and Obstetrics, West China Second University Hospital, Sichuan University, Chengdu 610041, People's Republic of China; Sichuan Key Laboratory of Gynecologic Oncology, West China Second University Hospital, Sichuan University, Chengdu 610041, People's Republic of China.
Biochem Biophys Res Commun. 2015 May 29;461(2):211-6. doi: 10.1016/j.bbrc.2015.02.171. Epub 2015 Apr 9.
In this work we aimed to identify the differentially expressed proteins and their potential roles during peri-implantation period through proteomics-based approach. Adult healthy female mice were mated naturally with fertile males to produce pregnancy. The models of pseudopregnancy, delayed implantation, and artificial decidualization were established. The protein profile between pre-implantation (D1) and implantation (D5) period was compared by two-dimensional electrophoresis (2-DE) and identified by mass spectrometry (MS). 2-DE yielded comparative images presenting over 500 protein spots in D1 and D5 mouse endometrium. 15 proteins were identified, of which stathmin 1, Apo-A1, hnRNP H3, transgelin 2 and arginase 1 were validated by western blotting. Stathmin 1 expression did not change in pseudopregnancy, but activation of implantation, or induction of decidualization increased it dramatically. Under non-pregnant status, progesterone alone or in combination with17β-estradiol increased it dramatically. Our results clarified the protein profile in mouse endometrium during implantation. The specific expression profile of stathmin 1 suggested that it should be involved in implantation and serve as a potential regulator of this process. These findings may contribute to the better understanding of the molecules events during embryo implantation, and subsequently improve the ability to treat infertility.
在本研究中，我们旨在通过基于蛋白质组学的方法，鉴定围着床期差异表达的蛋白质及其潜在作用。成年健康雌性小鼠与可育雄性自然交配以建立妊娠模型。建立了假孕、延迟着床和人工蜕膜化模型。通过二维电泳（2-DE）比较着床前（D1）和着床期（D5）的蛋白质图谱，并通过质谱（MS）进行鉴定。2-DE产生了比较图像，显示D1和D5小鼠子宫内膜中有500多个蛋白质斑点。鉴定出15种蛋白质，其中14-3-3σ、载脂蛋白A1、不均一核糖核蛋白H3、原肌球蛋白2和精氨酸酶1通过蛋白质免疫印迹法得到验证。14-3-3σ在假孕时表达不变，但着床激活或蜕膜化诱导时其表达显著增加。在非妊娠状态下，单独使用孕酮或与17β-雌二醇联合使用可显著增加其表达。我们的结果阐明了小鼠子宫内膜在着床期间的蛋白质图谱。14-3-3σ的特异性表达谱表明它可能参与着床过程，并作为这一过程的潜在调节因子。这些发现可能有助于更好地理解胚胎着床期间的分子事件，从而提高治疗不孕症的能力。
