Laboratório de Biomarcadores de Contaminação Aquática e Imunoquímica, Depto. Bioquímica, CCB, UFSC, Campus Trindade, 88040-900 Florianópolis, SC, Brazil; Laboratório de Ciências Marinhas, UNISUL, Av. Colombo Sales 89, 88790-000 Laguna, SC, Brazil.
Environ Toxicol Pharmacol. 2008 Nov;26(3):362-5. doi: 10.1016/j.etap.2008.05.004. Epub 2008 Jun 3.
Pacific oysters, Crassostrea gigas, were exposed to untreated sewage diluted in seawater. After 48h of exposure, the expression of genes associated to biotransformation pathways (CYP356A1, GSTO, MDR, FABP and ALAS) were analyzed in gills through semi-quantitative RT-PCR. A significant induction in all genes analyzed in the sewage-exposed oysters was observed. These genes are related to phase I (CYP356A1), phase II (GSTO) and phase III (MDR) biotransformation systems, to the uptake and transport of hydrophobic ligands (FABP) and to the synthesis of prosthetic group heme (ALAS). The organisms were able to survive in contaminated conditions since protective mechanisms have been properly stimulated.
太平洋牡蛎（Crassostrea gigas）暴露于未经处理的污水稀释海水中。暴露 48 小时后，通过半定量 RT-PCR 分析了鳃中与生物转化途径相关的基因（CYP356A1、GSTO、MDR、FABP 和 ALAS）的表达。在污水暴露的牡蛎中观察到所有分析基因均显著诱导。这些基因与 I 相（CYP356A1）、II 相（GSTO）和 III 相（MDR）生物转化系统、疏水性配体的摄取和转运（FABP）以及辅因子血红素的合成（ALAS）有关。由于适当刺激了保护机制，生物体能够在污染条件下存活。
