Flores-Nunes Fabrício, Mattos Jacó J, Zacchi Flávia L, Serrano Miguel A S, Piazza Clei E, Sasaki Silvio T, Taniguchi Satie, Bicego Márcia C, Melo Cláudio M R, Bainy Afonso C D
Laboratory of Biomarkers of Aquatic Contamination and Immunochemistry-LABCAI, Federal University Santa Catarina, Florianópolis, Brazil.
Aquaculture Pathology Research Center-NEPAQ, Federal University of Santa Catarina, Florianópolis, Brazil.
Environ Sci Pollut Res Int. 2015 Nov;22(22):17386-96. doi: 10.1007/s11356-015-4486-7. Epub 2015 Apr 14.
Urban effluents are rich in nutrients, organic matter, pharmaceuticals and personal care products (PPCPs), pesticides, hydrocarbons, surfactants, and others. Previous studies have shown that oysters Crassostrea gigas accumulate significant levels of linear alkylbenzenes (LABs) in sanitary sewage contaminated sites, but there is little information about its toxicological effects in marine bivalves. The aim of this study was to analyze the transcription of genes in two tissues of C. gigas exposed for 12, 24, and 36 h to LABs or sanitary sewage. Likewise, the activity of antioxidant and biotransformation enzymes was measured in oysters exposed for 36 h in all groups. Oysters exposed to LABs and oysters exposed to sanitary sewage showed different patterns of transcriptional responses. LAB-exposed oysters showed lower level of biological responses than the oysters exposed to sanitary sewage. Despite the ability of the oyster C. gigas to accumulate LABs (28-fold), the data indicate that these contaminants are not the cause for the transcriptional responses observed in oysters exposed to sanitary sewage. Possibly, the biological changes observed in the sanitary sewage-exposed oysters are associated with the presence of other contaminants, which might have caused synergistic, additive, or antagonistic effects. The results show that FABP-like and GST-ω-like messenger RNAs (mRNAs) have a rapid response in tissues of oyster C. gigas exposed to sanitary sewage, suggesting a possible protective response and a role in maintaining homeostasis of these organisms.
城市污水富含营养物质、有机物、药品和个人护理产品（PPCPs）、农药、碳氢化合物、表面活性剂等。先前的研究表明，太平洋牡蛎（Crassostrea gigas）在受生活污水污染的场地中会积累大量的直链烷基苯（LABs），但关于其对海洋双壳贝类的毒理学影响的信息却很少。本研究的目的是分析太平洋牡蛎暴露于LABs或生活污水12、24和36小时后，其两个组织中基因的转录情况。同样，还测量了所有组中暴露36小时的牡蛎的抗氧化酶和生物转化酶的活性。暴露于LABs的牡蛎和暴露于生活污水的牡蛎表现出不同的转录反应模式。暴露于LABs的牡蛎的生物反应水平低于暴露于生活污水的牡蛎。尽管太平洋牡蛎有积累LABs的能力（28倍），但数据表明这些污染物并不是导致暴露于生活污水的牡蛎中观察到的转录反应的原因。可能，在暴露于生活污水的牡蛎中观察到的生物学变化与其他污染物的存在有关，这些污染物可能产生了协同、相加或拮抗作用。结果表明，类脂肪酸结合蛋白（FABP-like）和类谷胱甘肽S-转移酶ω（GST-ω-like）信使核糖核酸（mRNAs）在暴露于生活污水的太平洋牡蛎组织中具有快速反应，表明可能存在保护反应以及在维持这些生物体内稳态方面的作用。
