大鼠胰腺中胰淀素的分泌及其在链脲佐菌素治疗后的选择性丧失。
Amylin secretion from the rat pancreas and its selective loss after streptozotocin treatment.
作者信息
Ogawa A, Harris V, McCorkle S K, Unger R H, Luskey K L
机构信息
Department of Internal Medicine, University of Texas Southwestern Medical Center, Dallas 75235.
出版信息
J Clin Invest. 1990 Mar;85(3):973-6. doi: 10.1172/JCI114528.
Abstract
Amylin, a peptide copackaged with insulin in beta-cell granules, was measured in the effluent of the perfused rat pancreases by means of a newly developed specific radioimmunoassay. Its secretion parallels that of insulin in response to 20 mM glucose, 10 mM arginine, or the combination thereof. The relative molar amount of secreted amylin was estimated to be 25-37% that of insulin. Treatment with a borderline diabetogenic dose of streptozotocin reduced amylin response without significantly changing the insulin response. A severely diabetogenic dose of streptozotocin totally abolished amylin release and markedly reduced insulin release. The selective impairment of amylin secretion in streptozotocin-treated rats could represent an early manifestation of beta-cell depletion or injury.
摘要
胰岛淀粉样多肽是一种与胰岛素共同包裹在β细胞颗粒中的肽，通过一种新开发的特异性放射免疫测定法，在灌注大鼠胰腺的流出物中对其进行了测量。在20 mM葡萄糖、10 mM精氨酸或二者组合的刺激下，其分泌与胰岛素的分泌情况相似。分泌的胰岛淀粉样多肽的相对摩尔量估计为胰岛素的25%-37%。用临界致糖尿病剂量的链脲佐菌素进行治疗可降低胰岛淀粉样多肽的反应，而不会显著改变胰岛素反应。高剂量致糖尿病剂量的链脲佐菌素则完全消除了胰岛淀粉样多肽的释放，并显著降低了胰岛素的释放。链脲佐菌素处理的大鼠中胰岛淀粉样多肽分泌的选择性受损可能是β细胞耗竭或损伤的早期表现。
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