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模型实验中两种从河流水环境浓缩病毒方法的效率比较

Comparison of the efficiency of two methods for virus concentration from river water environment in a model experiment.

作者信息

Wallnerová Z, Simková A

出版信息

J Hyg Epidemiol Microbiol Immunol. 1978;22(2):152-61.

PMID:217929
Abstract

Two of the methodical approaches for detection of virus contamination of surface waters were experimentally tested, i.e., the method of adsorption of virus particles to an inorganic salt (CaHPO4) and the ultrafiltration method using the Amicon apparatus. Under the given experimental conditions, the two methods showed practically identical factors of efficiency of virus concentration; the Danube river water proved to be a more suitable medium for the model of Coxsackie A4 virus than the physiological saline used as the control. In view of the feasibility, inexpensiveness and adequate effectivity of the precipitation method and with respect to good results obtained with it in field studies, the mentioned method of concentration could be recommended as the method of choice in field virological practice for aimed examination of surface waters.

摘要

对检测地表水病毒污染的两种系统方法进行了实验测试,即病毒颗粒吸附到无机盐(磷酸氢钙)上的方法和使用密理博仪器的超滤方法。在给定的实验条件下,这两种方法显示出几乎相同的病毒浓缩效率因子;事实证明,与用作对照的生理盐水相比,多瑙河水是柯萨奇A4病毒模型更合适的介质。鉴于沉淀法的可行性、低成本和足够的有效性,以及在现场研究中用该方法获得的良好结果,上述浓缩方法可推荐为现场病毒学实践中用于地表水针对性检测的首选方法。

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