Department of Inorganic and Analytical Chemistry, University of Debrecen, Debrecen, Hungary.
Electrophoresis. 2011 Aug;32(16):2223-8. doi: 10.1002/elps.201100185. Epub 2011 Jul 27.
MEKC with DAD was applied to detect six Gd-based contrasting agents (CAs) (Gd-DTPA-BMA (Omniscan), Gd-HPDO3A (ProHance), Gd-DOTA (Dotarem), Gd-AAZTA, Gd-BOPTA (Multihance) and Gd-DTPA (Magnevist)) commonly used in MRI diagnostics. The achieved LODs ranged between 0.40 and 20 μM and the optimized method gave excellent precision, especially when two internal standards were applied (less than 0.34 RSD% for migration time). The MEKC technique made it possible to determine the CAs in urine and serum samples of patients having a therapeutic dose. Due to the SDS content of the running buffer, the serum samples can be directly injected to analyze Gd-based CAs without interference of high protein content.
MEKC 与 DAD 联合应用于检测六种基于钆的造影剂（CAs）（Gd-DTPA-BMA（欧乃影）、Gd-HPDO3A（普罗海恩）、Gd-DOTA（多它醇）、Gd-AAZTA、Gd-BOPTA（马根维显）和 Gd-DTPA（美显醇）），这些造影剂常用于 MRI 诊断。所得到的检测限在 0.40 到 20μM 之间，优化后的方法具有极好的精密度，尤其是当使用两种内标时（迁移时间的 RSD%小于 0.34）。MEKC 技术使得能够在接受治疗剂量的患者的尿液和血清样本中测定 CAs。由于运行缓冲液中含有 SDS，因此可以直接注射血清样本来分析基于钆的 CAs，而不会受到高蛋白含量的干扰。
