Nottingham University Research and Treatment Unit in Reproduction (NURTURE), Division of Obstetrics and Gynaecology, School of Clinical Sciences, University of Nottingham, Nottingham, UK.
Ultrasound Obstet Gynecol. 2011 Nov;38(5):516-23. doi: 10.1002/uog.10046. Epub 2011 Oct 11.
To compare the reliability of our recently introduced technique for first-trimester embryo volume measurement, the 'semiautomated technique' using both Virtual Organ Computer-aided AnaLysis (VOCAL(™) ) and Sonography-based Automated Volume Count (SonoAVC) with a manual technique using VOCAL alone.
Seventy-four subjects with viable, singleton first-trimester in-vitro fertilization (IVF) pregnancies were recruited. Each subject underwent transvaginal sonography, at which a three-dimensional ultrasound dataset of the entire gestational sac was acquired. Each embryo volume was measured by two techniques, each performed twice. In the semiautomated technique VOCAL was used to calculate the volume of the gestational and yolk sacs, and SonoAVC was used to quantify the fluid volume within the amniotic and extra-amniotic cavities. Embryo volume was calculated by subtracting the sum of yolk sac, amniotic and extra-amniotic fluid volumes from gestational sac volume. In the manual technique, VOCAL was used to delineate the entire embryo using 9° rotations. Reliability was assessed using limits of agreement and intraclass correlation coefficient.
Datasets were included from 52 eligible subjects. Median gestational age was 7 + 4 weeks; median crown-rump length (CRL) was 13 (range, 2-29) mm; and median embryo volume was 1.8 (range, 0.03-8.1) cm(3) using the semiautomated technique and 0.7 (range 0.009-3.6) cm(3) using the manual technique. There was a significant discrepancy in the volumes measured by the two different techniques. Assessment of the limits of agreement suggested that the semiautomated technique (-15.8% to 14.0% of the mean embryo volume) was more reliable than was the manual technique (-22.4% to 26.6%).
The semiautomated technique is more reliable than is the manual technique for embryo volume measurement. However, the discrepancy in measurements between the two methods raises concerns over the validity of the semiautomated technique that require further investigation.
比较我们最近引入的用于测量早孕期胚胎体积的技术，即“半自动技术”（同时使用虚拟器官计算机辅助分析（VOCAL(™)）和基于超声的自动体积计数（SonoAVC））与单独使用 VOCAL 的手动技术的可靠性。
招募了 74 名具有可存活的单胎早孕期体外受精（IVF）妊娠的受试者。每位受试者均接受经阴道超声检查，获取整个孕囊的三维超声数据集。每个胚胎体积均通过两种技术测量两次。在半自动技术中，使用 VOCAL 计算孕囊和卵黄囊的体积，使用 SonoAVC 定量计算羊膜和羊膜外腔中的液体体积。胚胎体积通过从孕囊体积中减去卵黄囊、羊水和羊膜外液的总和来计算。在手动技术中，使用 VOCAL 以 9°旋转来描绘整个胚胎。使用一致性界限和组内相关系数评估可靠性。
从 52 名合格的受试者中纳入了数据集。中位孕龄为 7+4 周；中位头臀长（CRL）为 13（范围，2-29）mm；半自动技术测量的胚胎体积中位数为 1.8（范围，0.03-8.1）cm3，手动技术测量的胚胎体积中位数为 0.7（范围，0.009-3.6）cm3。两种不同技术测量的体积存在显著差异。对一致性界限的评估表明，半自动技术（平均胚胎体积的-15.8%至 14.0%）比手动技术（-22.4%至 26.6%）更可靠。
半自动技术比手动技术更可靠，用于胚胎体积测量。然而，两种方法之间的测量差异引起了对半自动技术有效性的关注，需要进一步调查。
Zotero 插件