The First Institute of Oceanography, Qingdao 266101, China.
J Environ Sci (China). 2011;23(4):693-8. doi: 10.1016/s1001-0742(10)60439-1.
We established an improved method for the determination of four estrogens including estriol (E3), 17beta-estradiol (E2), 17alpha-ethynylestrodiol (EE2) and estrone (El) in water. The method consisted of solid-phase extraction (0.5 L water) and subsequent analysis of analytes by ultra-performance liquid chromatography (UPLC) with an ultraviolet detector (UVD). Base-line separation was achieved for all studied estrogens using a column (50 mm x 2.1 mm) packed with 1.7 microm particle size stationary phase. Recovery was higher than 88% and detection limits ranged between 12.5-23.7 ng/L for the four estrogens, with the RSD ranging from 7% to 11%. The method was successfully applied to determine E2 and EE2 in simulated natural water, which found that about 70% of E2 was degraded (with a half-life of about 30 hr) within 48 hr and about 55% of EE2 was degraded (with a half-life of about 36 hr). Low levels of E1 were found, however E3 was undetectable during the process.
我们建立了一种改进的方法，用于测定水中的四种雌激素，包括雌三醇（E3）、17β-雌二醇（E2）、17α-乙炔基雌二醇（EE2）和雌酮（E1）。该方法包括固相萃取（0.5 L 水）和随后通过超高效液相色谱（UPLC）与紫外检测器（UVD）分析分析物。使用填充有 1.7 微米粒径固定相的柱（50mm×2.1mm）实现了所有研究的雌激素的基线分离。对于四种雌激素，回收率高于 88%，检测限在 12.5-23.7ng/L 之间，RSD 在 7%-11%之间。该方法成功应用于模拟天然水中的 E2 和 EE2 的测定，发现约 70%的 E2 在 48 小时内降解（半衰期约为 30 小时），约 55%的 EE2 降解（半衰期约为 36 小时）。在此过程中发现雌酮的水平较低，但无法检测到雌三醇。
