Banerjee Kaustuv, Michael Elizabeth, Eggink Dirk, van Montfort Thijs, Lasnik Amanda B, Palmer Kenneth E, Sanders Rogier W, Moore John P, Klasse Per Johan
Department of Microbiology and Immunology, Weill Cornell Medical College, New York, New York 10021, USA.
AIDS Res Hum Retroviruses. 2012 Feb;28(2):206-14. doi: 10.1089/aid.2011.0101. Epub 2011 Jul 27.
To assess the influence of mannosylated glycans on the immunogenicity of human immunodeficiency virus type 1 (HIV-1) Env proteins, we immunized mice with monomeric gp120 in the presence and absence of the mannose-binding protein, griffithsin (GRFT). For comparison, other groups of mice received the nonglycosylated HIV-1 Gag protein, with and without GRFT. Coimmunization with GRFT increased the anti-gp120 IgG reactivity significantly, but had no effect on the anti-Gag response. We also investigated the IgG response to GRFT and found that gp120, but not Gag, enhanced its immunogenicity. For both proteins, IgG1 antibodies dominated the IgG response, with IgG2b as the next most prevalent subclass. We conclude that gp120-GRFT complexes are more immunogenic than the free proteins, for both components, and that occluding the mannose moieties on monomeric gp120 can improve the humoral immune response to this protein.
为了评估甘露糖基化聚糖对1型人类免疫缺陷病毒（HIV-1）Env蛋白免疫原性的影响，我们在有和没有甘露糖结合蛋白——格里菲斯菌素（GRFT）存在的情况下，用单体gp120免疫小鼠。为作比较，其他几组小鼠分别接受了无糖基化的HIV-1 Gag蛋白，同样分为有和没有GRFT的情况。与GRFT共同免疫显著增加了抗gp120 IgG反应性，但对抗Gag反应没有影响。我们还研究了对GRFT的IgG反应，发现gp120而非Gag增强了其免疫原性。对于这两种蛋白，IgG1抗体在IgG反应中占主导，IgG2b是其次最普遍的亚类。我们得出结论，对于两种成分而言，gp120-GRFT复合物比游离蛋白更具免疫原性，并且封闭单体gp120上的甘露糖部分可以改善对该蛋白的体液免疫反应。
