The New York Eye and Ear Infirmary, New York, New York, USA.
Am J Ophthalmol. 2011 Nov;152(5):776-83.e1. doi: 10.1016/j.ajo.2011.04.031. Epub 2011 Jul 26.
To report pathologic evaluation and diagnostic yield of an aspiration cutter-assisted biopsy of anterior segment tumors.
Retrospective, consecutive, interventional case series.
Fifty-five eyes of 55 patients with iris and iridociliary tumors underwent an aspiration cutter-assisted biopsy at a single institution. Cytospin and cell-block preparations were performed on all biopsy samples. Bleached preparations and a panel of immunohistochemical stains were performed in selected cases. Cytologic diagnosis was correlated with clinical diagnosis and with histopathologic diagnosis, when available. Main outcome measures were (1) specimen cellularity, (2) diagnostic studies performed, (3) cytopathologic diagnosis, and (4) concordance with histopathologic diagnosis.
Specimen cellularity was adequate for cytopathologic interpretation of cytospin preparations in 55 (98.2%) of 56 biopsies. Twenty-three (41%) of 56 biopsy samples had diagnostic material in cell-block preparations. The most common cytopathologic diagnoses were melanoma (n = 39/56; 69.6%), melanocytoma (n = 4/56; 7.1%), nevus (n = 4/56; 7.1%), lymphoma (n = 2/56; 3.6%), and epithelial implantation cyst (n = 2/56; 3.6%). One biopsy sample (1.8%) yielded nondiagnostic material. Wide incisional or excisional biopsy confirmation was available in 13 (23.2%) of 56 aspiration cutter-assisted biopsy cases. Cytopathologic diagnoses were consistent with histopathologic diagnosis in 12 (92.3%) of 13 cases.
Although specialized pathologic techniques were necessary to maximize material available for diagnosis, all biopsies yielded cellular material and 41% yielded diagnostic tissue in cell block preparation. Although lower than the yield of wide incisional or excisional biopsy, aspiration cutter-assisted biopsy of anterior segment tumors achieved a diagnostic yield of 98.2%.
报告前段肿瘤抽吸切割辅助活检的病理评估和诊断率。
回顾性、连续、介入性病例系列。
在一家机构中，对 55 名患有虹膜和虹膜睫状体肿瘤的 55 只眼睛进行了抽吸切割辅助活检。对所有活检样本进行了细胞离心涂片和细胞块制备。在选定的病例中进行了漂白制备和免疫组织化学染色。细胞学诊断与临床诊断和组织病理学诊断相关联（如果有）。主要观察指标是：（1）标本细胞数量，（2）进行的诊断研究，（3）细胞病理学诊断，（4）与组织病理学诊断的一致性。
56 例活检中有 55 例（98.2%）的细胞离心涂片标本细胞数量足够进行细胞病理学解释。23 例（41%）的 56 例活检样本有细胞块制备的诊断材料。最常见的细胞学诊断是黑色素瘤（n = 39/56；69.6%）、黑色素细胞瘤（n = 4/56；7.1%）、痣（n = 4/56；7.1%）、淋巴瘤（n = 2/56；3.6%）和上皮植入囊肿（n = 2/56；3.6%）。1 例活检样本（1.8%）未获得诊断性材料。56 例抽吸切割辅助活检中有 13 例（23.2%）获得了广泛的切开活检或切除活检的确认。12 例（92.3%）的细胞学诊断与组织病理学诊断一致。
尽管需要专门的病理技术来最大限度地获取用于诊断的材料，但所有活检均获得了细胞材料，41%的活检在细胞块制备中获得了诊断性组织。尽管低于广泛切开活检或切除活检的诊断率，但前段肿瘤抽吸切割辅助活检的诊断率为 98.2%。
