Rozengurt N, Springall D R, Polak J M
Department of Veterinary Pathology, Royal Veterinary College, London, U.K.
J Pathol. 1990 Jan;160(1):5-8. doi: 10.1002/path.1711600104.
We have examined lungs from adult Wistar rats (n = 6) and four different strains of juvenile and adult mice (n = 40) to localize endothelin-like immunoreactivity. Paraffin sections of lung tissue fixed by distension in Bouin's fluid were stained by the peroxidase-antiperoxidase (PAP) method using 10 different rabbit antisera to endothelin. Immunoreactivity was detected in the majority of epithelial cells of conducting airways from the hilum to the periphery and was similar in rats and all four strains of mice studied. Intense immunostaining was detected in mucous, serous and Clara cells and in occasional alveolar pneumocytes type II. Basal cells and most ciliated cells did not immunostain. From these results it is concluded that endothelin-like immunoreactivity is present in bronchiolar epithelial cells in vivo in rats and mice.
我们检查了成年Wistar大鼠（n = 6）以及四种不同品系的幼年和成年小鼠（n = 40）的肺，以定位内皮素样免疫反应性。用Bouin氏液膨胀固定的肺组织石蜡切片，采用过氧化物酶-抗过氧化物酶（PAP）法，使用10种不同的抗内皮素兔抗血清进行染色。在从肺门到周边的传导气道的大多数上皮细胞中检测到免疫反应性，并且在所研究的大鼠和所有四种小鼠品系中相似。在粘液细胞、浆液细胞和克拉拉细胞以及偶尔的II型肺泡上皮细胞中检测到强烈的免疫染色。基底细胞和大多数纤毛细胞未显示免疫染色。从这些结果得出结论，在大鼠和小鼠体内，细支气管上皮细胞中存在内皮素样免疫反应性。
