Manabe T, Ikeda H, Moriya T, Yamashita K
Anat Rec. 1987 Feb;217(2):164-71. doi: 10.1002/ar.1092170208.
We used proteins in rat lung lavage fluid to successfully produce an antiserum against Clara cell secretory products. When used with the immunoperoxidase method, the antiserum specifically stained cells of the bronchiolar lining, which are morphologically consistent with Clara cells, as well as a few columnar cells in the bronchial and tracheal mucosa. B-5-fixed lung tissue furthermore demonstrated the immunoreactive layer over the bronchiolar epithelium. The alveolar and bronchial lining layers, on the other hand, were not immunoreactive, although a trace of granular immunoreactivity was seen in the latter. It was suggested that Clara cells produce and secrete some proteinaceous materials, which are mainly localized in the bronchiolar area after secretion and are seldom transferred into the alveolar lining layer. Our antibody cross-reacted with the Clara cells of mice, but not with those of hamsters, guinea pigs, rabbits, dogs, cats, monkeys, and man. The high degree of specificity of this antisera to Clara cells in formalin-fixed materials should make it a valuable tool for identifying Clara cell change in non-neoplastic lung pathology and in obtaining some insights into cell origin in neoplastic diseases.
我们使用大鼠肺灌洗液中的蛋白质成功制备了抗克拉拉细胞分泌产物的抗血清。当与免疫过氧化物酶法一起使用时，该抗血清特异性地染色细支气管内衬细胞，这些细胞在形态上与克拉拉细胞一致，以及支气管和气管黏膜中的一些柱状细胞。此外，B-5固定的肺组织显示细支气管上皮上有免疫反应层。另一方面，肺泡和支气管内衬层没有免疫反应，尽管在后者中可见微量颗粒状免疫反应。有人提出，克拉拉细胞产生并分泌一些蛋白质物质，这些物质分泌后主要定位于细支气管区域，很少转移到肺泡内衬层。我们的抗体与小鼠的克拉拉细胞发生交叉反应，但与仓鼠、豚鼠、兔子、狗、猫、猴子和人类的克拉拉细胞不发生交叉反应。这种抗血清对福尔马林固定材料中克拉拉细胞的高度特异性应使其成为识别非肿瘤性肺病理学中克拉拉细胞变化以及深入了解肿瘤性疾病细胞起源的有价值工具。
