Department of Biomedical Systems and Technologies, Nałęcz Institute of Biocybernetics and Biomedical Engineering, Polish Academy of Sciences, Warsaw, Poland.
Ann Biomed Eng. 2011 Nov;39(11):2721-34. doi: 10.1007/s10439-011-0366-6. Epub 2011 Jul 28.
The objectives were as follows: (1) estimating mean value of the overall hemoglobin glycation rate constant (k); (2) analyzing inter-individual variability of k; (3) verifying ability of the hemoglobin A1c (HbA1c) formation model to predict changes of HbA1c during red blood cells cultivation in vitro and to reproduce the clinical data. The mean k estimated in a group of 10 non-diabetic subjects was equal to 1.257 ± 0.114 × 10(-9) L mmol(-1) s(-1). The mean k was not affected by a way of estimation of glycemia. The mean k differed less than 20% from values reported earlier and it was almost identical to the mean values calculated on basis of the selected published data. Analysis of variability of k suggests that inter-individual heterogeneity of HbA1c formation is limited or rare. The HbA1c mathematical model was able to predict changes of HbA1c in vitro resulting from different glucose levels and to reproduce a linear relationship of HbA1c and average glucose obtained in the A1C-Derived Average Glucose Study. This study demonstrates that the glycation model with the same k value might be used in majority of individuals as a tool supporting interpretation of HbA1c in different clinical situations.
(1) 估算整体血红蛋白糖化率常数 (k) 的平均值；(2) 分析 k 的个体间变异性；(3) 验证血红蛋白 A1c (HbA1c) 形成模型预测体外培养红细胞中 HbA1c 变化的能力，并再现临床数据。在 10 名非糖尿病受试者的一组中，平均 k 值等于 1.257 ± 0.114×10(-9) L mmol(-1) s(-1)。k 的平均值不受血糖估计方式的影响。平均 k 值与之前报道的值相差小于 20%，与基于选定已发表数据计算的平均值几乎相同。k 变异性分析表明，HbA1c 形成的个体间异质性有限或罕见。HbA1c 数学模型能够预测体外不同葡萄糖水平引起的 HbA1c 变化，并再现 A1C-Derived Average Glucose 研究中获得的 HbA1c 与平均葡萄糖的线性关系。这项研究表明，对于大多数个体来说，具有相同 k 值的糖化模型可以作为一种工具，用于解释不同临床情况下的 HbA1c。
