[氧化亚铁硫杆菌核酮糖-1,5-二磷酸羧化酶/加氧酶基因的鉴定与表达]
[Identification and expression of ribulose 1,5-bisphosphate carboxylase/oxygenase gene from Thiobacillus versutus].
作者信息
Liu Z, Yan W
机构信息
State Key Laboratory of Microbial Technology, Shandong University, Jinan.
出版信息
Wei Sheng Wu Xue Bao. 1997 Aug;37(4):304-6.
PMID:9863205
Abstract
The chromosomal DNA of Thiobacillus versutus was hybridized with various heterologous ribulose 1,5-bisphosphate carboxylase/oxygenase (RubisCO) gene as probes. Only Rhodobacter sphaeroides form I RubisCO gene showed homology with T. versutus. The RubisCO gene fragment of T. versutus was isolated using the RubisCO gene of R. sphaeroides as a probe. And the RubisCO gene of T. versutus can express in E. coli cell.
摘要
用各种异源核酮糖-1,5-二磷酸羧化酶/加氧酶（RubisCO）基因作为探针，与维氏硫杆菌的染色体DNA进行杂交。只有球形红杆菌I型RubisCO基因与维氏硫杆菌显示出同源性。以球形红杆菌的RubisCO基因为探针，分离出维氏硫杆菌的RubisCO基因片段。并且维氏硫杆菌的RubisCO基因能在大肠杆菌细胞中表达。
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