Yu Ping, Sun Haisen, Li Jianrong, Wang Xiaohui, Lu Haixia
College of Food Science and Biotechnology, Zhejiang Gongshang University, Hangzhou 310035, China.
Wei Sheng Wu Xue Bao. 2011 Apr;51(4):561-5.
To explore the mechanism of cholesterol-degrading by Lactobacillus plantarum LpT1 and LpT2 in vitro.
After Lactobacillus plantarum LpT1 and LpT2 being inoculated and cultured in the medium MRS, MRS +CH, MRS + CH + S and MRS + CH + N, the cholesterol content in supernatant, deposit and cells, and the total cholesterol content before and after inoculation were determined and compared to primarily predict the mechanism of cholesterol-degrading by L. plantarum.
The mechanism of cholesterol- degrading by L. plantarum LpT1 and LpT2 in vitro involved in metabolic and non-metabolic pathway. Non-metabolic pathway was related to co-precipitation and cell absorption. Metabolic pathway was due to the production of the special enzymes during the growth of L. plantarum which made the cholesterol degrade to be other materials and caused its content reduction.
L. plantarum has shown its ability to degrade cholesterol in vitro.
探讨植物乳杆菌LpT1和LpT2体外降解胆固醇的机制。
将植物乳杆菌LpT1和LpT2接种于MRS、MRS + CH、MRS + CH + S和MRS + CH + N培养基中培养后，测定上清液、沉淀物和细胞中的胆固醇含量以及接种前后的总胆固醇含量并进行比较，初步预测植物乳杆菌降解胆固醇的机制。
植物乳杆菌LpT1和LpT2体外降解胆固醇的机制涉及代谢和非代谢途径。非代谢途径与共沉淀和细胞吸收有关。代谢途径是由于植物乳杆菌生长过程中产生特殊酶，使胆固醇降解为其他物质，导致其含量降低。
植物乳杆菌在体外已显示出降解胆固醇的能力。
