重组 CHIK 病毒 E1 外壳蛋白 11 kDa，具有抗原结构域，可用于检测基孔肯雅热。

Recombinant CHIK virus E1 coat protein of 11 KDa with antigenic domains for the detection of Chikungunya.

机构信息

SCMS Institute of Bioscience & Biotechnology Research & Development, Management House, South Kalamassery, Cochin, Kerala, PIN-682033, India.

出版信息

J Immunol Methods. 2011 Sep 30;372(1-2):171-6. doi: 10.1016/j.jim.2011.07.011. Epub 2011 Jul 21.

DOI:10.1016/j.jim.2011.07.011

PMID:21798263

Abstract

Chikungunya is an acute febrile illness caused by an alpha virus technically called as CHIK virus. A smaller size of CHIK virus E1 coat protein -11 kDa was expressed in prokaryotic expression system. The recombinant protein was purified and confirmed by western blot analysis. The positions of the antigenic domain in the protein were identified and the immunoreactivity of recombinant protein with anti-CHIK IgM antibodies was ascertained. The antigen showed an 88% sensitivity and 100% specificity by Indirect ELISA. No cross reactivity of the antigen was observed with anti-Dengue virus serum samples. The results strongly support that the recombinant CHIK coat protein could be used as a diagnostic antigen for the detection of Chikungunya by Indirect ELISA. The relevance of a smaller size recombinant antigen highlights its large scale application in serodiagnosis of CHIK virus since bacterial expression is more simple and cost effective than eukaryotic system.

摘要

基孔肯雅热是一种由 alpha 病毒引起的急性发热性疾病，技术上称为基孔肯雅病毒。在原核表达系统中表达了 CHIK 病毒 E1 外壳蛋白的较小尺寸 - 11 kDa。重组蛋白通过 Western blot 分析进行了纯化和确认。鉴定了该蛋白中抗原域的位置，并确定了重组蛋白与抗 CHIK IgM 抗体的免疫反应性。间接 ELISA 法显示该抗原的灵敏度为 88%，特异性为 100%。该抗原与抗登革热病毒血清样本无交叉反应。结果强烈支持重组 CHIK 外壳蛋白可作为间接 ELISA 检测基孔肯雅热的诊断抗原。较小尺寸重组抗原的相关性突出了其在基孔肯雅病毒血清学诊断中的大规模应用，因为细菌表达比真核系统更简单、更经济。