Cho Byungki, Kim Jungho, Cho Jang-Eun, Jeon Bo-Young, Park Sun
Standard Diagnostics Inc., Yongin 446-930, Republic of Korea.
J Virol Methods. 2008 Dec;154(1-2):154-9. doi: 10.1016/j.jviromet.2008.07.031. Epub 2008 Oct 1.
Chikungunya virus (CHIKV) causes endemic or epidemic outbreaks of CHIK fever, which typically manifests as a febrile illness. To develop a CHIKV-specific diagnostic test, CHIKV capsid protein was expressed using a baculovirus expression system. The seroreactvity of the recombinant CHIKV capsid protein was evaluated by ELISA and immuochromatographic assay (ICA), using 40 anti-CHIKV-positive and 20 anti-CHIKV-negative sera, an additional 20 normal sera samples from healthy Koreans, and 20 anti-Dengue virus sera samples. The sensitivity of the recombinant CHIKV capsid protein was 85% and 87.5% as measured by ELISA and ICA, respectively. The specificity of the recombinant CHIKV capsid protein was 100% both by ELISA and by ICA. No cross-reactivity of the capsid protein was seen with anti-Dengue virus sera samples. There was a significant correlation between the ELISA- and ICA-measured seroreactivities of the recombinant CHIKV capsid protein for anti-CHIKV IgM-positive sera samples. These results suggest that the recombinant CHIKV capsid protein could be used in a diagnostic test for identifying CHIKV disease.
基孔肯雅病毒（CHIKV）会引发基孔肯雅热的地方性或流行性疫情，该病通常表现为发热性疾病。为开发一种针对CHIKV的诊断测试，使用杆状病毒表达系统表达了CHIKV衣壳蛋白。采用酶联免疫吸附测定（ELISA）和免疫层析测定（ICA），使用40份抗CHIKV阳性血清、20份抗CHIKV阴性血清、另外20份来自健康韩国人的正常血清样本以及20份抗登革病毒血清样本，评估重组CHIKV衣壳蛋白的血清反应性。通过ELISA和ICA测定，重组CHIKV衣壳蛋白的敏感性分别为85%和87.5%。通过ELISA和ICA测定，重组CHIKV衣壳蛋白的特异性均为100%。未观察到衣壳蛋白与抗登革病毒血清样本有交叉反应。对于抗CHIKV IgM阳性血清样本，重组CHIKV衣壳蛋白的ELISA和ICA测定血清反应性之间存在显著相关性。这些结果表明，重组CHIKV衣壳蛋白可用于CHIKV疾病的诊断测试。
