Rianthavorn Pornpimol, Wuttirattanakowit Norra, Prianantathavorn Kesmanee, Limpaphayom Noppachart, Theamboonlers Apiradee, Poovorawan Yong
Center of Excellence in Clinical Virology, Department of Pediatrics, Faculty of Medicine, Chulalongkorn University, Bangkok, Thailand.
Southeast Asian J Trop Med Public Health. 2010 Jan;41(1):92-6.
Chikungunya (CHIK) is a re-emerging disease causing a large negative impact on global health and economics. Clinical manifestations of CHIK are non specific and difficult to differentiate from dengue hemorrhagic fever or other viral exanthema. A rapid, simple and reliable diagnostic assay is necessary for CHIK outbreak control especially in countries with insufficient access to well-equipped laboratories. The aim of the study is to evaluate a commercially rapid, qualitative CHIK diagnostic assay based on specific IgM antibody detection. Performance of the rapid assay was evaluated in comparison with semi-nested RT-PCR and IgM detection by ELISA. The sensitivity of the rapid assay was not constant but positively correlated with duration of symptoms. If the test was conducted within the first week, sensitivity and specificity was 22% and 88%, respectively. If the patients were tested after the first week, sensitivity was increased to 83% while specificity was decreased to 71%. Thus, the rapid assay should not be used as a screening tool during the first week of CHIK due to its low sensitivity.
基孔肯雅热（CHIK）是一种再度出现的疾病，对全球健康和经济造成了巨大的负面影响。基孔肯雅热的临床表现不具特异性，难以与登革出血热或其他病毒疹相区分。对于控制基孔肯雅热疫情，尤其是在缺乏设备完善实验室的国家，一种快速、简单且可靠的诊断检测方法是必要的。本研究的目的是评估一种基于特异性IgM抗体检测的商业化快速定性基孔肯雅热诊断检测方法。将该快速检测方法的性能与半巢式RT-PCR以及ELISA法检测IgM的性能进行了比较评估。快速检测方法的灵敏度并非恒定不变，而是与症状持续时间呈正相关。如果在第一周内进行检测，灵敏度和特异性分别为22%和88%。如果在第一周后对患者进行检测，灵敏度提高到83%，而特异性降至71%。因此，由于其灵敏度较低，在基孔肯雅热发病的第一周内，快速检测方法不应用作筛查工具。
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