Prain C J, Ross C A
Centre for Tropical Veterinary Medicine, Easter Bush, Roslin, Midlothian.
Parasitology. 1990 Feb;100 Pt 1:107-13.
Differentiation of epimastigotes and production of infective metacyclic forms of Trypanosoma congolense were examined in a culture system which enabled manipulation of the population density of insect forms. Scanning electron microscopy of cultures revealed the attachment sites of epimastigotes in detail, showing them to be attached as 'clusters' or 'bundles' and having associated fibrillar structures. Dividing epimastigotes were observed either within individual bundles or in association with two bundles. Metacyclic forms were detected by an immunofluorescence antibody test (IFAT) using metacyclic variable-antigen type (M-VAT) specific monoclonal antibodies, by day 7 after seeding cultures. Trypanosomes expressing M-VATs appeared singly in bundles, observed by both IFAT and an immunogold labelling method. Statistical analysis using Poisson calculations suggested that, in general, the distribution of metacyclics expressing individual M-VATs was random throughout cultures.
在一个能够控制昆虫型群体密度的培养系统中，研究了刚果锥虫前鞭毛体的分化和感染性循环后期形式的产生。培养物的扫描电子显微镜详细揭示了前鞭毛体的附着位点，显示它们以“簇”或“束”的形式附着，并具有相关的纤维状结构。在单个束内或与两个束相关联处观察到正在分裂的前鞭毛体。接种培养物7天后，通过使用循环后期可变抗原类型（M-VAT）特异性单克隆抗体的免疫荧光抗体试验（IFAT）检测到循环后期形式。通过IFAT和免疫金标记方法观察到，表达M-VAT的锥虫单独出现在束中。使用泊松计算的统计分析表明，一般来说，表达单个M-VAT的循环后期形式在整个培养物中的分布是随机的。
