Ross C A
Centre for Tropical Veterinary Medicine, University of Edinburgh, Midlothian, Scotland.
Acta Trop. 1987 Sep;44(3):293-301.
The effect of glutamine on the number of metacyclic trypanosomes produced in insect form cultures of Trypanosoma congolense TREU 1457 was investigated. When cultured in the absence of glutamine, trypanosomes did not develop to metacyclic forms. While metacyclics were produced in all cultures maintained with glutamine in the concentration range 2-20 mM, optimum numbers were obtained between 4 and 12 mM glutamine. This pattern was constant in flasks cultured for over two months. The substitution of glutamine by proline in culture medium did not influence the number of metacyclic forms produced. Removal of proline or glutamine from cultures producing metacyclics caused a rapid fall in production of metacyclic forms which could be reversed by re-introduction of either nutrient. Cultures of another stock of T. congolense responded similarly when maintained in different concentrations of glutamine, showing that the effect may be a general one.
研究了谷氨酰胺对刚果锥虫TREU 1457昆虫型培养物中产生的循环后期锥虫数量的影响。在无谷氨酰胺的情况下培养时，锥虫不会发育成循环后期形式。当在浓度范围为2-20 mM的谷氨酰胺维持的所有培养物中产生循环后期锥虫时，在4-12 mM谷氨酰胺之间可获得最佳数量。这种模式在培养超过两个月的烧瓶中是恒定的。在培养基中用脯氨酸替代谷氨酰胺不会影响产生的循环后期形式的数量。从产生循环后期锥虫的培养物中去除脯氨酸或谷氨酰胺会导致循环后期形式的产量迅速下降，重新引入任何一种营养物质都可以使其逆转。当在不同浓度的谷氨酰胺中维持时，另一株刚果锥虫的培养物也有类似反应，表明这种影响可能是普遍的。
