Department of Veterinary Infectious Diseases, College of Veterinary Medicine, Chonnam National University, Gwangju, Republic of Korea.
Cancer Gene Ther. 2011 Sep;18(9):646-54. doi: 10.1038/cgt.2011.36. Epub 2011 Jul 29.
Interferon-gamma (IFN-γ) exhibits biological activities that are considered to have important roles in tumor suppression. Therefore, the IFN-γ gene is a potential candidate for in vivo cytokine gene therapy against skin cancer. The present study evaluated the efficacy of a hydrodynamics-based IFN-γ gene transfection for skin cancer treatment, in which the plasmid DNA encoding IFN-γ was administered into the tail vein of mice following 7,12-dimethylbenz[a]anthracene and 12-O-tetradecanoylphorbol-13-acetate-induced skin carcinogenesis. Serum levels of IFN-γ were substantially elevated without liver toxicity. The mice injected with IFN-γ plasmid DNA displayed a marked reduction in papilloma numbers, suppressed proliferation of epidermal cells and induction of caspase-3-mediated apoptosis. These results suggest that the hydrodynamics-based transfection of IFN-γ plasmid DNA is a convenient and efficient means of skin cancer gene therapy.
干扰素-γ（IFN-γ）具有生物活性，被认为在肿瘤抑制中具有重要作用。因此，IFN-γ 基因是体内细胞因子基因治疗皮肤癌的潜在候选基因。本研究评估了基于流体动力学的 IFN-γ 基因转染治疗皮肤癌的疗效，其中在二甲基苯并蒽（7,12-DMBA）和 12-O-十四烷酰佛波醇-13-醋酸盐诱导的皮肤癌变后，将编码 IFN-γ 的质粒 DNA 经尾静脉注入小鼠。IFN-γ 质粒 DNA 注射的小鼠血清 IFN-γ 水平显著升高，且无肝毒性。注射 IFN-γ 质粒 DNA 的小鼠皮肤肿瘤数量明显减少，表皮细胞增殖受到抑制，诱导 caspase-3 介导的细胞凋亡。这些结果表明，基于流体动力学的 IFN-γ 质粒 DNA 转染是一种方便有效的皮肤癌基因治疗方法。
