Glazunov A V
Radiobiologiia. 1990 Jan-Feb;30(1):3-15.
The role of DNA double-strand break (DSB) repair in radioresistance of Saccharomyces cerevisiae G1 cells is discussed. The contribution of rapid and slow DNA DSB repair to radioresistance of diploid yeast has been estimated. The contribution of the DNA DSB repair involving no homologous chromosome interaction is shown to be insignificant in comparison with the recombinational repair. The rapid DNA DSB repair efficiency calculation method based on the proposed yeast radiation inactivation model is given. The calculations are in a satisfactory agreement with the experimental data. Possible mechanisms of radiation induction of lethal sectoring in yeast are discussed. This phenomenon is supposed to be due to the DNA DSB processing during vegetative division of irradiated cells. A general scheme of radiation inactivation of yeast cells is proposed.
讨论了DNA双链断裂（DSB）修复在酿酒酵母G1期细胞辐射抗性中的作用。评估了快速和慢速DNA DSB修复对二倍体酵母辐射抗性的贡献。结果表明，与重组修复相比，不涉及同源染色体相互作用的DNA DSB修复的贡献微不足道。给出了基于所提出的酵母辐射失活模型的快速DNA DSB修复效率计算方法。计算结果与实验数据吻合良好。讨论了酵母中致死扇形化辐射诱导的可能机制。推测这种现象是由于受辐照细胞营养分裂过程中的DNA DSB处理所致。提出了酵母细胞辐射失活的总体方案。
