Xiao Rong-ping, Huang Jian-dong, Zhang Han-hui
College of Chemistry and Chemical Engineering, Fuzhou University, Fuzhou 350108, China.
Guang Pu Xue Yu Guang Pu Fen Xi. 2011 May;31(5):1322-7.
Four kinds of covalent serum albumin (BSA or HSA) conjugates of beta-tetrakis[(3,5-dicarboxy)phenoxy]-phthalocyaninatozinc (1) and beta-octakis [(3, 5-dicarboxy) phenoxy]-phthalocyaninatozinc (2) were synthesized by the approach of amide bond. The molar ratio of phthalocyanine to albumin in conjugates were found to be 6 - 7.1. Their absorption spectra were measured in PBS solution. When conjugated to albumin, compound 1 displays more distinct monomer absorption characteristics (with the maximum absorption at 677 nm) than its free form. Compound 2 in albumin framework exists mostly in monomer form, which is beneficial to photodynamic therapy in aqueous solution. Both 2-BSA and 2-HSA show a sharp and intense Q-band at 681 nm and 682 nm with the molar extinction coefficient of 2.01 x 10(5) and 2.05 x 10(5) mol(-1) L cm(-1) respectively. The Q-band absorption spectra and existent state of phthalocyanine 1 or 2 in conjugates were not affected by the pH value of aqueous solution, which are obviously different from that of the corresponding free phthalocyanine.
通过酰胺键合方法合成了四种β-四[（3,5-二羧基）苯氧基] - 酞菁锌（1）和β-八[（3,5-二羧基）苯氧基] - 酞菁锌（2）与共价血清白蛋白（牛血清白蛋白或人血清白蛋白）的缀合物。发现缀合物中酞菁与白蛋白的摩尔比为6 - 7.1。在磷酸盐缓冲盐溶液（PBS）中测量了它们的吸收光谱。与白蛋白缀合时，化合物1与其游离形式相比表现出更明显的单体吸收特性（最大吸收波长为677 nm）。白蛋白框架中的化合物2大多以单体形式存在，这有利于在水溶液中的光动力治疗。2-牛血清白蛋白（2-BSA）和2-人血清白蛋白（2-HSA）在681 nm和682 nm处均显示出尖锐且强烈的Q带，摩尔消光系数分别为2.01×10⁵和2.05×10⁵ mol⁻¹ L cm⁻¹。缀合物中酞菁1或2的Q带吸收光谱和存在状态不受水溶液pH值的影响，这与相应的游离酞菁明显不同。
