Wasser M N, Pauwels E K, Nieuwenhuizen W
Gaubius Institute TNO, Leiden, The Netherlands.
Thromb Res Suppl. 1990;10:91-104. doi: 10.1016/0049-3848(90)90383-n.
This paper describes the production and characterization of an antifibrin monoclonal antibody, produced by immunizing mice with fibrinogen degradation fragment Y. The antibody (designated Y22) is directed against a conformation dependent epitope in the D-domain of fibrin Y22 was labeled with the radionuclide Tc-99m. Experiments in vitro and in rats are presented which show the potential of scintigraphic detection of thrombi with Tc-99m-Y22. Preliminary results show that immunoscintigraphy of thrombi may also have potential for the monitoring of thrombolytic therapy (eg with t-PA).
本文描述了一种抗纤维蛋白单克隆抗体的制备及其特性，该抗体是通过用纤维蛋白原降解片段Y免疫小鼠产生的。该抗体（命名为Y22）针对纤维蛋白D结构域中一个依赖构象的表位。Y22用放射性核素锝-99m进行标记。文中展示了在体外和大鼠体内进行的实验，这些实验表明锝-99m-Y22对血栓进行闪烁扫描检测的潜力。初步结果表明，血栓免疫闪烁扫描在监测溶栓治疗（如使用组织型纤溶酶原激活剂）方面也可能具有潜力。
