Knight L C, Maurer A H, Ammar I A, Epps L A, Dean R T, Pak K Y, Berger H J
Department of Diagnostic Imaging, Temple University School of Medicine and Hospital, Philadelphia, PA 19140.
Radiology. 1989 Oct;173(1):163-9. doi: 10.1148/radiology.173.1.2781002.
An antifibrin antibody (T2G1s) Fab' fragment labeled with technetium-99m was tested for its ability to produce images of fresh thrombi in dogs. In gamma camera images, all thrombi were evident by 2-4 hours after injection. Mean thrombus-to-blood and thrombus-to-muscle ratios averaged 4.0 and 69 at four hours after injection and increased to 24 and 270, respectively, by 24 hours after injection. When compared with I-125 fibrinogen injected into the same dogs, Tc-99m-antifibrin Fab' had lower absolute uptake in thrombus but higher thrombus-to-blood ratios due to a faster rate of disappearance from the blood. The primary route of excretion was through the kidneys. Tc-99m-antifibrin Fab' was highly stable in vivo, with an average of 82% of the circulating radioactivity able to bind to fibrin at 4 hours after injection. When compared with an In-111-labeled Fab fragment of antifibrin antibody 59D8, thrombus-to-blood and thrombus-to-muscle ratios were slightly higher for the Tc-99m-labeled antibody, and the blood disappearance rate was slightly faster. The absolute uptake in thrombus, however, was not significantly different, and the thrombus was visualized at about the same time after injection. These studies suggest that Tc-99m T2G1s Fab' is a potential agent for detecting thrombi in a clinical setting.
对一种用锝-99m标记的抗纤维蛋白抗体（T2G1s）Fab片段进行了检测，以评估其在犬体内生成新鲜血栓图像的能力。在γ相机图像中，注射后2 - 4小时所有血栓均清晰可见。注射后4小时，血栓与血液以及血栓与肌肉的平均比值分别为4.0和69，到注射后24小时分别增至24和270。与注射到同一只犬体内的碘-125纤维蛋白原相比，锝-99m抗纤维蛋白Fab片段在血栓中的绝对摄取量较低，但由于从血液中消失的速度较快，血栓与血液的比值较高。排泄的主要途径是通过肾脏。锝-99m抗纤维蛋白Fab片段在体内高度稳定，注射后4小时平均有82%的循环放射性能够与纤维蛋白结合。与抗纤维蛋白抗体59D8的铟-111标记Fab片段相比，锝-99m标记抗体的血栓与血液以及血栓与肌肉的比值略高，血液消失速度略快。然而，在血栓中的绝对摄取量无显著差异，注射后血栓在大致相同的时间显影。这些研究表明，锝-99m T2G1s Fab片段是临床环境中检测血栓的一种潜在试剂。
