Vieira D C M, Salgado H R N
Programa de Pós-graduação em Ciências Farmacêuticas, Faculdade de Ciências Farmacêuticas de Araraquara UNESP, Rodovia AraraquaraJaú, km 1, CEP 14801-902, Araraquara SP, Brazil.
J Chromatogr Sci. 2011 Aug;49(7):508-11. doi: 10.1093/chrsci/49.7.508.
This paper describes the development and evaluation of a HPLC and UV spectrophotometric methods to quantify cefuroxime sodium in injectables. HPLC analysis were carried out using a C18 Wat 054275 column and a mobile phase composed of methanol and water (70:30), with a flow rate of 0.8 mL/min and UV detection at 280 nm. For the spectrophotometric analysis, water was used as solvent and the wavelength of 280 nm was selected for the detection. Both methods were found to quantify cefuroxime sodium in injectables accurately. Therefore HPLC and UV methods presented the most reliable results for the analyses of injectables.
本文描述了一种用于定量注射剂中头孢呋辛钠的高效液相色谱法（HPLC）和紫外分光光度法的开发与评估。HPLC分析使用C18 Wat 054275色谱柱，流动相由甲醇和水（70:30）组成，流速为0.8 mL/min，在280 nm处进行紫外检测。对于分光光度分析，以水为溶剂，选择280 nm波长进行检测。发现这两种方法都能准确地定量注射剂中的头孢呋辛钠。因此，HPLC和紫外方法在注射剂分析中给出了最可靠的结果。
