Department of Biochemistry & Biotechnology, Annamalai University, Tamil Nadu, India.
Redox Rep. 2011;16(3):91-100. doi: 10.1179/174329211X13020951739839.
The status of lipid peroxidation, antioxidants, and detoxification enzymes were used as biochemical end points to assess the chemopreventive potential of geraniol, a monoterpene, in 7,12-dimethylbenz[a]anthracene (DMBA)-induced hamster buccal pouch carcinogenesis. Topical application of 0.5% DMBA in liquid paraffin, three times a week, for 14 weeks developed well-differentiated squamous cell carcinoma in the buccal pouch of golden Syrian hamsters. Although 100% tumor formation was noticed in hamsters treated with DMBA alone, intragastric administration of geraniol, at a dose of 250 mg/kg body weight (b.w.) to DMBA-treated hamster completely prevented the formation of oral tumors. Furthermore, geraniol significantly reduced lipid peroxidation by-products and improved the status of enzymatic and non-enzymatic antioxidants as well as modulated the status of phase I and phase II detoxification enzymes, favoring the excretion of carcinogenic metabolite, during DMBA-induced oral carcinogenesis. The present study concludes that the chemopreventive potential of geraniol relies on its anti-lipid peroxidative and antioxidant function as well as modulatory effects on phase I and II detoxification enzymes to excrete the carcinogenic metabolite, during DMBA-induced hamster buccal pouch carcinogenesis.
脂质过氧化、抗氧化剂和解毒酶的状态被用作生化终点，以评估香叶醇（一种单萜）在 7,12-二甲基苯并[a]蒽（DMBA）诱导的金黄地鼠颊囊癌变中的化学预防潜力。每周三次在液体石蜡中局部应用 0.5%的 DMBA 持续 14 周，可在金黄地鼠颊囊中诱导出分化良好的鳞状细胞癌。尽管单独用 DMBA 处理的仓鼠中观察到 100%的肿瘤形成，但用 250mg/kg 体重（b.w.）的香叶醇灌胃处理 DMBA 处理的仓鼠可完全阻止口腔肿瘤的形成。此外，香叶醇显著减少了脂质过氧化产物，并改善了酶和非酶抗氧化剂的状态，以及调节了 I 相和 II 相解毒酶的状态，有利于致癌代谢物的排泄，在 DMBA 诱导的口腔癌变过程中。本研究得出结论，香叶醇的化学预防潜力依赖于其抗脂质过氧化和抗氧化功能，以及对 I 相和 II 相解毒酶的调节作用，以排出致癌代谢物，在 DMBA 诱导的金黄地鼠颊囊癌变过程中。
