Department of Biological Sciences, University of Alberta, Edmonton, AB, Canada.
Dev Comp Immunol. 2012 Jan;36(1):199-207. doi: 10.1016/j.dci.2011.07.005. Epub 2011 Jul 21.
Granulocyte colony stimulating factor receptor (GCSFR) is a member of the class I cytokine receptor superfamily and signaling through this receptor is important for the proliferation, differentiation and activation of neutrophils and their precursors. In this study we report on the cloning and molecular characterization of goldfish GCSFR. The identified goldfish GCSFR sequence possesses the conserved Ig-like domain, the cytokine receptor homology domain (CRH), three fibronectin domains as well as several intracellular signaling motifs characteristic of other vertebrate GCSFRs. Goldfish gcsfr mRNA was highly expressed in kidney and spleen, and in primary kidney neutrophils. The neutrophils have significantly higher mRNA levels of the transcription factors pu.1 and cebpα, and down-regulated levels of transcription factors important for macrophage development such as egr1 and cjun, compared to progenitor cells from the kidney. The gcsfr mRNA was present in the kidney progenitor cells, albeit at much lower levels compared to the neutrophils, and the expression of gcsfr in progenitor cells was not affected by duration of cultivation. Furthermore, gcsfr mRNA levels were up-regulated in neutrophils after treatment with heat-killed Aeromonas salmonicida A449 or with mitogens. Our results indicate that GCSFR may be a useful marker for fish neutrophils.
粒细胞集落刺激因子受体 (GCSFR) 是 I 类细胞因子受体超家族的成员，通过该受体的信号转导对于中性粒细胞及其前体细胞的增殖、分化和激活非常重要。在这项研究中，我们报告了金鱼 GCSFR 的克隆和分子特征。鉴定出的金鱼 GCSFR 序列具有保守的 Ig 样结构域、细胞因子受体同源结构域 (CRH)、三个纤维连接蛋白结构域以及几个与其他脊椎动物 GCSFR 特征一致的细胞内信号转导基序。金鱼 gcsfr mRNA 在肾脏和脾脏以及原代肾脏中性粒细胞中高度表达。与祖细胞相比，中性粒细胞中转录因子 pu.1 和 cebpα 的 mRNA 水平显著升高，而对巨噬细胞发育很重要的转录因子 egr1 和 cjun 的水平下调。与中性粒细胞相比，gcsfr mRNA 存在于肾脏祖细胞中，但水平要低得多，并且祖细胞中 gcsfr 的表达不受培养时间的影响。此外，用热灭活的鲑鱼气单胞菌 A449 或有丝分裂原处理后，中性粒细胞中的 gcsfr mRNA 水平上调。我们的结果表明，GCSFR 可能是鱼类中性粒细胞的有用标志物。
