Katzenback Barbara A, Belosevic Miodrag
Department of Biological Sciences, University of Alberta, Edmonton, Alberta, T6G 2E9 Canada.
Dev Comp Immunol. 2009 Apr;33(4):601-11. doi: 10.1016/j.dci.2008.10.011. Epub 2008 Nov 29.
Neutrophils are one of the first cells to arrive at the site of inflammation and are central cells of innate immunity of vertebrates. Upon activation, neutrophils are capable of producing an intense respiratory burst response, they rapidly degranulate in the presence of pathogens, and deploy neutrophil extracellular traps that efficiently remove infections agents. We report on the isolation, cultivation and characterization of neutrophil-like cells isolated from the goldfish kidney. Neutrophil-like cells stained positive for acid phosphatase and Sudan black, expressed MPO, and produced a dose-dependent respiratory burst response after stimulation with phorbol esters (PMA) and calcium ionophore A23187 (CaI). Treatment of neutrophil-like cultures with PMA, CaI or zymosan induced a dose-dependent non-reversible degranulation of neutrophil-like cells. Goldfish neutrophil-like cells also exhibited a chemotactic response, degranulation, and respiratory burst response when exposed to the virulent fish pathogen, Aeromonas salmonicida A449.
中性粒细胞是最早抵达炎症部位的细胞之一，是脊椎动物固有免疫的核心细胞。激活后，中性粒细胞能够产生强烈的呼吸爆发反应，在病原体存在的情况下迅速脱颗粒，并部署中性粒细胞胞外陷阱以有效清除感染因子。我们报告了从金鱼肾脏分离出的类中性粒细胞的分离、培养和特性。类中性粒细胞酸性磷酸酶和苏丹黑染色呈阳性，表达髓过氧化物酶（MPO），在用佛波酯（PMA）和钙离子载体A23187（CaI）刺激后产生剂量依赖性呼吸爆发反应。用PMA、CaI或酵母聚糖处理类中性粒细胞培养物会诱导类中性粒细胞发生剂量依赖性的不可逆脱颗粒。当暴露于毒性鱼类病原体杀鲑气单胞菌A449时，金鱼类中性粒细胞也表现出趋化反应、脱颗粒和呼吸爆发反应。
