Cronican James J, Beier Kevin T, Davis Tina N, Tseng Jen-Chieh, Li Weida, Thompson David B, Shih Allen F, May Erin M, Cepko Constance L, Kung Andrew L, Zhou Qiao, Liu David R
Howard Hughes Medical Institute, Department of Chemistry and Chemical Biology, Harvard University, 12 Oxford Street, Cambridge, MA 02138, USA.
Chem Biol. 2011 Jul 29;18(7):833-8. doi: 10.1016/j.chembiol.2011.07.003.
We discovered a class of naturally occurring human proteins with unusually high net positive charge that can potently deliver proteins in functional form into mammalian cells both in vitro and also in murine retina, pancreas, and white adipose tissues in vivo. These findings represent diverse macromolecule delivery agents for in vivo applications, and also raise the possibility that some of these human proteins may penetrate cells as part of their native biological functions.
我们发现了一类天然存在的人类蛋白质，其净正电荷异常高，能够在体外以及在小鼠视网膜、胰腺和白色脂肪组织的体内实验中，以功能形式有效地将蛋白质递送至哺乳动物细胞中。这些发现代表了用于体内应用的多种大分子递送剂，也增加了一些人类蛋白质可能作为其天然生物学功能的一部分穿透细胞的可能性。
