单分子荧光共振能量转移揭示了两个甲基修饰对人线粒体赖氨酸转运RNA（tRNA(Lys)）折叠的协同作用。

Single-molecule FRET reveals a cooperative effect of two methyl group modifications in the folding of human mitochondrial tRNA(Lys).

作者信息

Kobitski Andrei Yu, Hengesbach Martin, Seidu-Larry Salifu, Dammertz Kirsten, Chow Christine S, van Aerschot Arthur, Nienhaus G Ulrich, Helm Mark

机构信息

Institute of Applied Physics and Center for Functional Nanostructures, Karlsruhe Institute of Technology, 76131 Karlsruhe, Germany.

出版信息

Chem Biol. 2011 Jul 29;18(7):928-36. doi: 10.1016/j.chembiol.2011.03.016.

DOI:10.1016/j.chembiol.2011.03.016

PMID:21802013

Abstract

Using a combination of advanced RNA synthesis techniques and single molecule spectroscopy, the deconvolution of individual contributions of posttranscriptional modifications to the overall folding and stabilization of human mitochondrial tRNA(Lys) is described. An unexpected destabilizing effect of two pseudouridines on the native tRNA folding was evidenced. Furthermore, the presence of m(2)G10 alone does not facilitate the folding of tRNA(Lys), but a stabilization of the biologically functional cloverleaf shape in conjunction with the principal stabilizing component m(1)A9 exceeds the contribution of m(1)A alone. This constitutes an unprecedented cooperative effect of two nucleotide modifications in the context of a naturally occurring RNA, which may be of general importance for tRNA structure and help understanding several recently described decay pathways for hypomodified tRNAs.

摘要

利用先进的RNA合成技术和单分子光谱学相结合的方法，描述了转录后修饰对人线粒体tRNA（Lys）整体折叠和稳定性的个体贡献的去卷积。证实了两个假尿苷对天然tRNA折叠具有意想不到的不稳定作用。此外，单独存在m（2）G10并不能促进tRNA（Lys）的折叠，但与主要稳定成分m（1）A9一起，生物功能三叶草形状的稳定性超过了单独m（1）A的贡献。这构成了天然存在的RNA背景下两种核苷酸修饰前所未有的协同效应，这可能对tRNA结构具有普遍重要性，并有助于理解最近描述的几种低修饰tRNA的降解途径。