Spenkuch Felix, Motorin Yuri, Helm Mark
a Institute of Pharmacy and Biochemistry ; Johannes Gutenberg-University of Mainz ; Mainz , Germany.
RNA Biol. 2014;11(12):1540-54. doi: 10.4161/15476286.2014.992278.
Pseudouridine (Ψ) is the most abundant of >150 nucleoside modifications in RNA. Although Ψ was discovered as the first modified nucleoside more than half a century ago, neither the enzymatic mechanism of its formation, nor the function of this modification are fully elucidated. We present the consistent picture of Ψ synthases, their substrates and their substrate positions in model organisms of all domains of life as it has emerged to date and point out the challenges that remain concerning higher eukaryotes and the elucidation of the enzymatic mechanism.
假尿苷（Ψ）是RNA中150多种核苷修饰中最丰富的一种。尽管半个多世纪前Ψ就作为第一种修饰核苷被发现，但它的形成酶促机制及其修饰功能均未得到充分阐明。我们展示了假尿苷合酶、其底物及其在生命所有领域的模式生物中的底物位置的一致情况，这是迄今为止所呈现的，并指出了关于高等真核生物以及酶促机制阐明方面仍然存在的挑战。
