Heiman H S, Weisman L E
Department of Pediatrics, Uniformed Services University, Bethesda, MD 20814-4799.
Vet Immunol Immunopathol. 1990 Jan;24(1):79-89. doi: 10.1016/0165-2427(90)90079-8.
Group B streptococcus (GBS) remains a major cause of neonatal infection. Maternal immunization-induced GBS antibody may protect neonates from GBS disease. Since the opsonophagocytosis assay correlates well with survival in GBS infected suckling rats, we sought to determine an immunization schedule which would induce type III GBS opsonic antibody in rat dams above a predetermined level of 10 dilution-1 (dil-1). This schedule could then be used for future studies of maternal-fetal immunity. Wistar rat dams (n = 12) were given killed GBS type III using three immunization schedules (primary injection, initial booster at 7, 14 or 22 days and then weekly boosters). Opsonic GBS type III antibody and total immunoglobulin (IgG) were measured. Only the schedule with a 7-day initial booster resulted in GBS type-specific opsonic antibody consistently above 10 dil-1. The IgG (467 +/- 83 mg/100 ml) remained constant while the opsonic antibody increased significantly to 50 and 63 dil-1 (P less than 0.01 compared to day 0) after boosters on day 7 and 14 respectively. Eight pregnant dams, who received a primary immunization and boosters at 7 and 14 days, developed GBS type III opsonic antibody titers (72 dil-1) similar to non-pregnant dams and potentially adequate to protect suckling rats from GBS disease. This model may now be used to study other adjuvants, immunogens, and maternal-fetal immunity using established suckling rat models of GBS disease.
B族链球菌（GBS）仍然是新生儿感染的主要原因。母体免疫诱导产生的GBS抗体可能会保护新生儿免受GBS疾病的侵害。由于吞噬调理作用试验与GBS感染的乳鼠存活率密切相关，我们试图确定一种免疫方案，该方案能使大鼠母体内III型GBS调理抗体诱导至预定水平10倍稀释度-1（dil-1）以上。然后该方案可用于未来母婴免疫的研究。将12只Wistar大鼠母鼠分为三组，采用三种免疫方案（初次注射，分别在第7、14或22天进行初次加强免疫，之后每周进行一次加强免疫）给予灭活的III型GBS。检测III型GBS调理抗体和总免疫球蛋白（IgG）。只有初次加强免疫在第7天的方案能使GBS型特异性调理抗体持续高于10 dil-1。IgG（467±83 mg/100 ml）保持恒定，而调理抗体在第7天和第14天加强免疫后分别显著增加至50和63 dil-1（与第0天相比，P<0.01）。8只在第0天进行初次免疫，并在第7天和第14天进行加强免疫的怀孕母鼠，其III型GBS调理抗体滴度（72 dil-1）与未怀孕母鼠相似，可能足以保护乳鼠免受GBS疾病的侵害。现在可以使用这个模型，利用已建立的GBS疾病乳鼠模型来研究其他佐剂、免疫原和母婴免疫。
