Chengdu Institute of Biology, Chinese Academy of Sciences, Chengdu 610041, PR China.
Virology. 2011 Sep 1;417(2):248-52. doi: 10.1016/j.virol.2011.06.020. Epub 2011 Jul 29.
Long-terminal repeat (LTR) retrotransposons typically contain gag, pol, or gag-pol, and in some case env genes. In this work, we used data mining of the Botrytis cinerea genomic sequence and a molecular approach to identify Boty-like LTR retrotransposons in B. cinerea containing an antisense gene (brtn) between pol and the 3'-LTR. Reverse transcriptase PCR (RT-PCR) revealed that some brtn-like genes could be expressed, at least in B. cinerea T4. We conducted BLAST comparisons and conserved-domain analysis, but the function of putative BRTN is presently unknown. Boty-like LTR retrotransposons in Sclerotinia sclerotiorum, called ScscLRET and containing brtn homologs at positions similar to brtn, were detected by homology searches and data mining of the S. sclerotiorum 1980 genomic sequence. Thus, this study demonstrated that some fungal LTR retrotransposons contain additional antisense genes.
长末端重复（LTR）反转录转座子通常包含 gag、pol 或 gag-pol，在某些情况下还包含 env 基因。在这项工作中，我们使用博落回内生真菌基因组序列的数据挖掘和分子方法，鉴定了博落回内生真菌中的 Boty 样 LTR 反转录转座子，其 pol 和 3'-LTR 之间含有反义基因（brtn）。逆转录 PCR（RT-PCR）显示，一些 brtn 样基因至少可以在博落回内生真菌 T4 中表达。我们进行了 BLAST 比较和保守结构域分析，但目前尚不清楚假定的 BRTN 的功能。通过同源搜索和对 Sclerotinia sclerotiorum 1980 基因组序列的数据挖掘，检测到 Sclerotinia sclerotiorum 中的 Boty 样 LTR 反转录转座子，称为 ScscLRET，其位置与 brtn 类似，含有 brtn 同源物。因此，本研究表明，一些真菌 LTR 反转录转座子含有额外的反义基因。
