Diolez A, Marches F, Fortini D, Brygoo Y
Institut National de la Recherche Agronomique, Versailles, France.
Appl Environ Microbiol. 1995 Jan;61(1):103-8. doi: 10.1128/aem.61.1.103-108.1995.
The phytopathogenic fungus Botrytis cinerea can infect an extremely wide range of host plants (tomato, grapevine, strawberry, and flax) without apparent specialization. While studying genetic diversity in this fungus, we found an element which is present in multiple copies and dispersed throughout the genome of some of its isolates. DNA sequence analysis revealed that the element contained direct, long-terminal repeats (LTRs) of 596 bp whose features were characteristic of retroviral and retrotransposon LTRs. Within the element, we identified an open reading frame with sequences homologous to the reverse transcriptase and RNase H domains of retroelement pol genes. We concluded that the element we had identified was a retroelement and named it Boty. By comparing its open reading frame with sequences from other retroelements, we found that Boty is related to the gypsy family of retrotransposons. Boty was present in numerous strains isolated from grapes and tomatoes but not in isolates from lentils. We propose that Boty-containing and Boty-deficient groups represent two lineages in the population of B. cinerea.
植物病原真菌灰葡萄孢能够感染范围极其广泛的寄主植物（番茄、葡萄、草莓和亚麻），且无明显的寄主专化性。在研究这种真菌的遗传多样性时，我们发现了一种元件，它以多个拷贝存在，并分散在其一些分离株的基因组中。DNA序列分析表明，该元件包含596 bp的直接长末端重复序列（LTRs），其特征是逆转录病毒和反转录转座子LTRs的特征。在该元件内，我们鉴定出一个开放阅读框，其序列与反转录元件pol基因的逆转录酶和核糖核酸酶H结构域同源。我们得出结论，我们鉴定出的元件是一个反转录元件，并将其命名为Boty。通过将其开放阅读框与其他反转录元件的序列进行比较，我们发现Boty与反转录转座子的吉普赛家族有关。Boty存在于从葡萄和番茄中分离出的众多菌株中，但不存在于从扁豆中分离出的菌株中。我们认为，含有Boty和缺乏Boty的群体代表了灰葡萄孢种群中的两个谱系。
