Department of Obstetrics, Perinatal Pharmacology, University Hospital Zurich, Zurich, Switzerland.
Phytomedicine. 2011 Dec 15;19(1):74-82. doi: 10.1016/j.phymed.2011.06.032. Epub 2011 Jul 30.
The use of preparations from Bryophyllum pinnatum (Lamarck) Oken (Kalanchoe pinnata (Lamarck) Persoon) in tocolysis is supported by clinical evidence. We studied here the effect of B. pinnatum leaf press juice and its chemical fractions on the response of human myometrial strips. No data are available if the influence on myometrial strips of the juice differs from that of its components in the chemical fractions, in order to increase the pharmacological effect.
In vitro study to test the effect of repeated addition of B. pinnatum leaf press juice (BPJ) and its chemical components in several dilutions (undiluted, 1-10%) on myometrium strips hang up in a myograph chamber. Chemical analysis is including HPLC, MPLC with Sephadex LH-20 and TLC.
All test solutions are inhibiting contractility by reducing the amplitude and the area under the curve (AUC) of the contractions. Undiluted BPJ and its undiluted chemical fraction 4 are reducing most effective these two parameters: the amplitude was at 78% of the baseline (95% CI (77-89); p<0.05) at the second addition of the BPJ and at 70% (95% CI (50-90); p<0.05) of the first addition of fraction 4; the AUC was at 82% (95% CI (69-95); p<0.05) of the baseline at the first addition of the press juice and at 51% (95% CI (27-74); p<0.05) at the first addition of fraction 4. The BPJ decreased amplitude and AUC significantly faster and increased frequency significantly faster than the control. Fractions could be tentatively assigned to bufadienolids, flavonoids and cinnamic acids. Fraction 4, accounted for flavonoids, increased the frequency of the contractions most effectively: 557% of the baseline (95% CI (316-797); p<0.05) at the first addition.
Leaf juice of B. pinnatum and its flavonoid fraction are most effective in relaxing myometrial strips by inducing frequency.
临床证据表明，使用落地生根（Bryophyllum pinnatum（Lamarck）Oken）制剂进行保胎治疗是有效的。本研究旨在探讨落地生根叶压榨汁及其化学馏分对人子宫平滑肌条反应的影响。目前尚无数据表明压榨汁对子宫平滑肌条的影响是否与其化学馏分中的成分不同，因为这可以增加药理作用。
在体外研究中，我们测试了重复加入落地生根叶压榨汁（BPJ）及其化学组分在几个稀释度（未稀释、1-10%）对悬挂在肌描记器室中的子宫平滑肌条的影响。化学分析包括 HPLC、MPLC 与 Sephadex LH-20 和 TLC。
所有测试溶液均通过减少收缩幅度和曲线下面积（AUC）来抑制收缩力。未稀释的 BPJ 和其未稀释的化学馏分 4 对这两个参数的抑制作用最为明显：在第二次加入 BPJ 时，幅度为基础值的 78%（95%置信区间（77-89）；p<0.05），在第一次加入 4 馏分时为 70%（95%置信区间（50-90）；p<0.05）；在第一次加入压榨汁时，AUC 为基础值的 82%（95%置信区间（69-95）；p<0.05），在第一次加入 4 馏分时为 51%（95%置信区间（27-74）；p<0.05）。BPJ 降低幅度和 AUC 的速度明显快于对照组，增加频率的速度也明显快于对照组。馏分可初步分配到蟾毒配基、黄酮类化合物和肉桂酸类化合物。黄酮类化合物组成的馏分 4 对收缩频率的影响最为明显：第一次加入时为基础值的 557%（95%置信区间（316-797）；p<0.05）。
落地生根叶汁及其黄酮类馏分通过诱导频率对子宫平滑肌条最有效。
