用于胺类和蛋白质的明亮荧光紫和蓝色标签。

Brightly fluorescent purple and blue labels for amines and proteins.

机构信息

Institute of Analytical Chemistry, Chemo- and Biosensors, University of Regensburg, D-93053 Regensburg, Germany.

出版信息

Bioorg Med Chem Lett. 2011 Sep 15;21(18):5538-42. doi: 10.1016/j.bmcl.2011.06.133. Epub 2011 Jul 13.

DOI:10.1016/j.bmcl.2011.06.133

Abstract

Novel amino-reactive phenoxazines were obtained by reasonably simple synthetic protocols and characterized in terms of their use as fluorescent labels for amines, amino acids and proteins in general. Purple labels (alternatives to Texas Red) and blue labels (alternatives to Cy-1) were obtained by this strategy. The absorption/emission maxima, in aqueous solution, are at around 589/630 nm and 648/670 nm, respectively, thus indicating larger Stokes' shifts than those of common cyanine-type of labels. The new labels are compatible with commercial diode laser light sources.

摘要

新型氨基反应性呫吨通过合理简单的合成方案获得，并根据其在一般胺、氨基酸和蛋白质的荧光标记中的用途进行了表征。通过该策略获得了紫色标签（Texas Red 的替代品）和蓝色标签（Cy-1 的替代品）。在水溶液中的吸收/发射最大值分别约为 589/630nm 和 648/670nm，这表明其斯托克斯位移比常见的菁染料类型的标记物更大。新标签与商业二极管激光光源兼容。