INRA, AgroParisTech, Institut Jean-Pierre Bourgin, UMR 1318, F-78000 Versailles, France.
J Plant Physiol. 2011 Nov 15;168(17):2015-20. doi: 10.1016/j.jplph.2011.06.007. Epub 2011 Jul 31.
Despite the importance of seed oil bodies (OBs) as enclosed compartments for oil storage, little is known about lipid and protein accumulation in OBs during seed formation. OBs from rapeseed (Brassica napus) consist of a triacylglycerol (TAG) core surrounded by a phospholipid monolayer embedded with integral proteins which confer high stability to OBs in the mature dry seed. In the present study, we investigated lipid and protein accumulation patterns throughout seed development (from 5 to 65 days after pollination [DAP]) both in the whole seed and in purified OBs. Deposition of the major proteins (oleosins, caleosins and steroleosins) into OBs was assessed through (i) gene expression pattern, (ii) proteomics analysis, and (iii) protein immunodetection. For the first time, a sequential deposition of integral OB proteins was established. Accumulation of oleosins and caleosins was observed starting from early stages of seed development (12-17 DAP), while steroleosins accumulated later (~25 DAP) onwards.
尽管种子油体 (OBs) 作为油脂储存的封闭隔室非常重要，但对于种子形成过程中 OBs 中的脂质和蛋白质积累知之甚少。油菜籽 (Brassica napus) 的 OBs 由三酰基甘油 (TAG) 核心组成，周围是一层嵌入整合蛋白的磷脂单层，这些整合蛋白赋予 OBs 在成熟干燥种子中的高稳定性。在本研究中，我们在整个种子和纯化的 OBs 中研究了从授粉后 5 天到 65 天（DAP）的种子发育过程中的脂质和蛋白质积累模式。通过 (i) 基因表达模式、(ii) 蛋白质组学分析和 (iii) 蛋白质免疫检测来评估主要蛋白质（油体蛋白、钙蛋白和甾醇蛋白）向 OBs 的沉积情况。首次建立了完整 OB 蛋白的顺序沉积。从种子发育的早期阶段（12-17 DAP）开始观察到油体蛋白和钙蛋白的积累，而甾醇蛋白则在稍后（~25 DAP）积累。
